Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.26.4 extracted from

  • Amon, J.D.; Koshland, D.
    RNase H enables efficient repair of R-loop induced DNA damage (2016), eLife, 5, e20533 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.4

Protein Variants

Protein Variants Comment Organism
additional information generation of the rnh1DELTA rnh201DELTA mutant with disrupted activity of the two enzymes RNase H1 and H, kinetics of foci persistence in rnh1D rnh201D cells, phenotype, overview. Cell cycle delay is observed in the rnh1DELTA rnh201DELTA strain and in rnh1DELTA rnh201DELTA TOP1-AID cells. The Rad52-GFP foci in the rnh1DELTA rnh201DELTA double mutant accumulated in a window that begins at the boundary between S and G2-M. Screen for suppressors of hybrid-induced lethality, identifying Pif1-E467G, this allele suppresses auxin sensitivity when introduced into rnh1DELTA rnh201DELTA TOP1-AID cells, indicating its responsibility for the suppression of lethality in the mutant cells. Pif1 mutants inhibit break-induced replication. RPA190 mutants allow for repair of R-loop induced damage. Rpa190-K1482T and -V1486F suppress auxin sensitivity of rnh1DELTA rnh201DELTA TOP1-AID cells Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Saccharomyces cerevisiae 5634
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P53942
-
-
Saccharomyces cerevisiae Q04740
-
-

Synonyms

Synonyms Comment Organism
RNase H1
-
 Saccharomyces cerevisiae
RNase H2
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction disrupting the activity of the two enzymes RNase H1 and H2 (rnh1DELTA rnh201DELTA in Saccharomyces cerevisiae) is a useful tool for increasing the persistence of DNA:RNA hybrids and studying the effects of hybrid-induced instability. In the absence of RNase H activity, the levels of hybrids formed at susceptible loci increase dramatically. This increase in hybrids is associated with increased rates of genome instability that include loss of heterozygosity (LOH) events, loss of entire chromosomes, and recombination at the ribosomal locus. rnh1DELTA rnh201DELTA mutants display an increase in Rad52-GFP foci. Cells lacking RNase H1 and H2 have a larger fraction of persistent R-loop induced damage than wild-type cells or cells lacking only one of the RNases H, failure to observe accumulating foci early in the cell cycle, phenotype, overview Saccharomyces cerevisiae
physiological function R-loops are structures that form when RNA invades double-stranded DNA and hybridizes to complementary genomic sequences. R-loops can form spontaneously across many genomic loci, but the activity of two endogenous RNases H prevents their accumulation and persistence. RNase H enables efficient repair of R-loop induced DNA damage. RNase H1 and H2 are highly conserved ribonucleases with the ability to degrade the RNA moiety of a DNA:RNA hybrid. The RNases H are important protectors of genome stability, mechanisms, overview. The presence of either RNase H1 or H2 prevents the accumulation of DNA damage in G2-M Saccharomyces cerevisiae