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Literature summary for 3.1.26.4 extracted from

  • Tannous, E.; Yokoyama, K.; You, D.J.; Koga, Y.; Kanaya, S.
    A dual role of divalent metal ions in catalysis and folding of RNase H1 from extreme halophilic archaeon Halobacterium sp. NRC-1 (2012), FEBS Open Bio, 2, 345-352.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus(DE3) cells Halobacterium salinarum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme requires either salt or divalent metal ions for folding. The enzyme exhibits activity in the presence of divalent metal ions regardless of the presence or absence of 3 M NaCl. However, higher concentrations of divalent metal ions are required for activity in the absence of salt to facilitate folding Halobacterium salinarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 x * 24000, SDS-PAGE Halobacterium salinarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RNA-DNA hybrid + H2O Halobacterium salinarum
-
 ribonucleotide 5'-phosphomonoester + ?
-
 ?
RNA-DNA hybrid + H2O Halobacterium salinarum NRC 1
-
 ribonucleotide 5'-phosphomonoester + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Halobacterium salinarum Q9HSF6
-
-
Halobacterium salinarum NRC 1 Q9HSF6
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Q column chromatography, hydroxyapatite column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration Halobacterium salinarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA-DNA hybrid + H2O
-
 Halobacterium salinarum ribonucleotide 5'-phosphomonoester + ?
-
 ?
RNA-DNA hybrid + H2O
-
 Halobacterium salinarum NRC 1 ribonucleotide 5'-phosphomonoester + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 24000, SDS-PAGE Halobacterium salinarum

Synonyms

Synonyms Comment Organism
RNase H1
-
 Halobacterium salinarum
RNH1
-
 Halobacterium salinarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
46.2 76.5 the melting temperature in the presence of 300 mM MgCl2, 20 mM MnCl2, 3 M NaCl, or 300 mM MgCl2 plus 3 M NaCl is 46.2°C, 50°C, 66.9°C, and 76.5°C, respectively Halobacterium salinarum