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Literature summary for 3.1.26.4 extracted from
- Structure-specific nuclease activities of Pyrococcus abyssi RNase HII (2010), J. Bacteriol., 192, 3689-3698.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene PAB0352 | Pyrococcus abyssi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | a 5' RNA flap Okazaki fragment intermediate impairs PabRNase HII endonuclease activity. Introduction of mismatches into the RNA portion near the RNA-DNA junction decreases both the specificity and the efficiency of cleavage by PabRNase HII | Pyrococcus abyssi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
RNA-DNA/DNA hybrid | pH 8.0, 37°C, purified recombinant His-tagged enzyme | Pyrococcus abyssi |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | the type 2 RNase H is an Mg2+ and alkaline pH-dependent enzyme | Pyrococcus abyssi |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNA-DNA/DNA hybrid + H2O | Pyrococcus abyssi | PabRNase HII acts as a specific endonuclease on RNA-DNA/DNA duplexes. Specific cleavage, one nucleotide upstream of the RNA-DNA junction, occurs on a substrate in which RNA initiators is fully annealed to the cDNA template. Additionally, PabRNase HII cleaves a single ribonucleotide embedded in a double-stranded DNA | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus abyssi | - |
gene PAB0352 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNA-DNA/DNA hybrid + H2O | PabRNase HII acts as a specific endonuclease on RNA-DNA/DNA duplexes. Specific cleavage, one nucleotide upstream of the RNA-DNA junction, occurs on a substrate in which RNA initiators is fully annealed to the cDNA template. Additionally, PabRNase HII cleaves a single ribonucleotide embedded in a double-stranded DNA | Pyrococcus abyssi | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase HII | an archaeal type 2 RNase H | Pyrococcus abyssi |
| type 2 RNase H | - |
Pyrococcus abyssi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Pyrococcus abyssi |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.093 | - |
RNA-DNA/DNA hybrid | pH 8.0, 37°C, purified recombinant His-tagged enzyme | Pyrococcus abyssi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the type 2 RNase H is an Mg2+- and alkaline pH-dependent enzyme | Pyrococcus abyssi |
| 8 | - |
assay at | Pyrococcus abyssi |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the type 2 RNase H is an Mg2+- and alkaline pH-dependent enzyme | Pyrococcus abyssi |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 185.7 | - |
RNA-DNA/DNA hybrid | pH 8.0, 37°C, purified recombinant His-tagged enzyme | Pyrococcus abyssi |
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