Cloned (Comment) | Organism |
---|---|
genetic organization of the RNase H1-related gene family, different isozymes by alternative splicing, sequence comparisons of isozymes, overexpression of His-tagged isozymes in Escherichia coli strain BL21(DE3) | Caenorhabditis elegans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of isozymes | Caenorhabditis elegans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | a divalent metal ion is required, dependent on the isozyme | Caenorhabditis elegans | |
Mn2+ | a divalent metal ion is required, dependent on the isozyme | Caenorhabditis elegans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
different splicing variants | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozymes to near homogeneity from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Caenorhabditis elegans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | developmental stage-specific expression from L3 to adult stage of RNAse H1 isozymes with different substrate specificities and divalent cation requirements, overview | Caenorhabditis elegans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.057 | - |
isozyme RNase H1beta, with Mn2+ | Caenorhabditis elegans |
0.07 | - |
isozyme RNase H1beta, with Mg2+ | Caenorhabditis elegans |
2.1 | - |
isozyme RNase H1alpha, with Mn2+ | Caenorhabditis elegans |
7 | - |
isozyme RNase H1alpha, with Mg2+ | Caenorhabditis elegans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
M13 DNA-RNA hybrid + H2O | the enzyme degrades the RNA moiety of the heteroduplex | Caenorhabditis elegans | ? | - |
? | |
additional information | stage-specific expression of RNAse H1 isozymes with different substrate specificities and divalent cation requirements, claevage specificties, overview | Caenorhabditis elegans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease H1 | - |
Caenorhabditis elegans |
RNase H1 | - |
Caenorhabditis elegans |