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Literature summary for 3.1.26.4 extracted from

  • Kochiwa, H.; Itaya, M.; Tomita, M.; Kanai, A.
    Stage-specific expression of Caenorhabditis elegans ribonuclease H1 enzymes with different substrate specificities and bivalent cation requirements (2006), FEBS J., 273, 420-429.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genetic organization of the RNase H1-related gene family, different isozymes by alternative splicing, sequence comparisons of isozymes, overexpression of His-tagged isozymes in Escherichia coli strain BL21(DE3) Caenorhabditis elegans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of isozymes Caenorhabditis elegans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ a divalent metal ion is required, dependent on the isozyme Caenorhabditis elegans
Mn2+ a divalent metal ion is required, dependent on the isozyme Caenorhabditis elegans

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
different splicing variants
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozymes to near homogeneity from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Caenorhabditis elegans

Source Tissue

Source Tissue Comment Organism Textmining
additional information developmental stage-specific expression from L3 to adult stage of RNAse H1 isozymes with different substrate specificities and divalent cation requirements, overview Caenorhabditis elegans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.057
-
isozyme RNase H1beta, with Mn2+ Caenorhabditis elegans
0.07
-
isozyme RNase H1beta, with Mg2+ Caenorhabditis elegans
2.1
-
isozyme RNase H1alpha, with Mn2+ Caenorhabditis elegans
7
-
isozyme RNase H1alpha, with Mg2+ Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
M13 DNA-RNA hybrid + H2O the enzyme degrades the RNA moiety of the heteroduplex Caenorhabditis elegans ?
-
?
additional information stage-specific expression of RNAse H1 isozymes with different substrate specificities and divalent cation requirements, claevage specificties, overview Caenorhabditis elegans ?
-
?

Synonyms

Synonyms Comment Organism
ribonuclease H1
-
Caenorhabditis elegans
RNase H1
-
Caenorhabditis elegans