Cloned (Comment) | Organism |
---|---|
gene rnhB, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain MIC2067(DE3), a rnhA/rnhB double mutant strain, as 60% soluble and 40% insoluble protein | Shewanella sp. |
General Stability | Organism |
---|---|
denaturation kinetics, midpoint at about 1.6 M urea and 5°C | Shewanella sp. |
denaturation kinetics, midpoint at about 1.8 M urea and 5°C | Escherichia coli |
metal ion and substrate stabilize the enzyme | Escherichia coli |
metal ion and substrate stabilize the enzyme | Shewanella sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.071 | - |
M13 DNA-RNA hybrid | pH 8.5, 15°C, recombinant enzyme | Shewanella sp. | |
0.075 | - |
M13 DNA-RNA hybrid | pH 8.5, 30°C, recombinant enzyme | Shewanella sp. | |
0.26 | - |
M13 DNA-RNA hybrid | pH 8.5, 15°C or 30°C, recombinant enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Escherichia coli | |
Co2+ | activates, best at 0.5 mM | Shewanella sp. | |
KCl | activates best at 110 mM, preferred to NaCl | Shewanella sp. | |
KCl | equally activating as NaCl | Escherichia coli | |
Mg2+ | activates | Escherichia coli | |
Mg2+ | activates, best at 5 mM | Shewanella sp. | |
Mn2+ | activates | Escherichia coli | |
Mn2+ | activates, best at 1 mM, strongly preferred divalent cation | Shewanella sp. | |
additional information | no activation by Ca2+, Zn2+, Ba2+, Ni2+, Cu2+, Fe2+, and Sr2+ | Escherichia coli | |
additional information | no activation by Ca2+, Zn2+, Ba2+, Ni2+, Cu2+, Fe2+, and Sr2+ | Shewanella sp. | |
NaCl | activates best at 60 mM | Shewanella sp. | |
NaCl | equally activating as KCl | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
recombinant enzyme, gel filtration | Shewanella sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P10442 | gene rnhB | - |
Shewanella sp. | Q25C12 | psychrotrophic bacterium, gene rnhB | - |
Shewanella sp. SIB1 | Q25C12 | psychrotrophic bacterium, gene rnhB | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain MIC2067(DE3) to homogeneity by heparin affinity chromatography and gel filtration | Shewanella sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.31 | - |
15°C, pH 8.5, purified recombinant enzyme | Escherichia coli |
1.1 | - |
30°C, pH 8.5, purified recombinant enzyme | Escherichia coli |
7.6 | - |
15°C, pH 8.5, purified recombinant enzyme | Shewanella sp. |
22 | - |
30°C, pH 8.5, purified recombinant enzyme | Shewanella sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-RNA hybrid + H2O | - |
Escherichia coli | ? | - |
? | |
DNA-RNA hybrid + H2O | - |
Shewanella sp. | ? | - |
? | |
DNA-RNA hybrid + H2O | - |
Shewanella sp. SIB1 | ? | - |
? | |
M13 DNA-RNA hybrid + H2O | - |
Escherichia coli | ? | - |
? | |
M13 DNA-RNA hybrid + H2O | - |
Shewanella sp. | ? | - |
? | |
M13 DNA-RNA hybrid + H2O | - |
Shewanella sp. SIB1 | ? | - |
? | |
additional information | substrate cleavage mode of the enzyme, cleavage site specificity | Escherichia coli | ? | - |
? | |
additional information | substrate cleavage mode of the enzyme, cleavage site specificity | Shewanella sp. | ? | - |
? | |
additional information | substrate cleavage mode of the enzyme, cleavage site specificity | Shewanella sp. SIB1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 25000, recombinant enzyme, SDS-PAGE | Shewanella sp. |
Synonyms | Comment | Organism |
---|---|---|
RNase HII | - |
Escherichia coli |
RNase HII | - |
Shewanella sp. |
RnhB | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Shewanella sp. |
45 | - |
- |
Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 60 | - |
Escherichia coli |
15 | 60 | - |
Shewanella sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
half-life about 30 min | Shewanella sp. |
30 | - |
half-life about 45 min | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Escherichia coli |
10 | - |
- |
Shewanella sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.1 | 12 | - |
Escherichia coli |
7.1 | 12 | - |
Shewanella sp. |