Literature summary for 3.1.26.4 extracted from

Banks, G.R.
A ribonuclease H from Ustilago maydis. Properties, mode of action and substrate specificity of the enzyme (1974), Eur. J. Biochem., 47, 499-507.

Search for more literature for 3.1.26.4

Activating Compound

Activating Compound	Comment	Organism	Structure
(NH4)2SO4	4fold stimulation at an optimal concentration of 18 mM	Ustilago maydis
2-mercaptoethanol	stimulates	Ustilago maydis

Inhibitors

Inhibitors	Comment	Organism	Structure
(NH4)2SO4	above 60 mM	Ustilago maydis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Ustilago maydis
Mn2+	Mn2+ or Mg2+ required	Ustilago maydis
Mn2+	optimal concentration: 1.2 mM	Ustilago maydis
NaCl	stimulates	Ustilago maydis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
100000	130000	gel filtration	Ustilago maydis

Organism

Organism	UniProt	Comment	Textmining
Ustilago maydis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Ustilago maydis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Ustilago maydis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
RNA-DNA hybrid + H2O	can degrade about 90% of the RNA strand of and RNA-DNA hybrid	Ustilago maydis	ribonucleotide 5'-phosphomonoester	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
32	-	-	Ustilago maydis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	8	Tris buffer or potassium phosphate buffer	Ustilago maydis

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Release 2023.1 (January 2023)