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Literature summary for 3.1.26.3 extracted from

  • Comeau, M.A.; Lafontaine, D.A.; Abou Elela, S.
    The catalytic efficiency of yeast ribonuclease III depends on substrate specific product release rate (2016), Nucleic Acids Res., 44, 7911-7921 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.3

Cloned(Commentary)

Cloned (Comment) Organism
gene RNT1, recombinant expression Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
additional information product inhibition, presence of 800 nM products reduces the value of the initial phase kcat by 5fold. The reduction of Rnt1p activity by the product is impaired when the reaction is carried out with a buffer containing 500 mM KCl, which reduces the stability of Rnt1p/RNA complex. Addition of Xrn1p, which degrades the reaction products, to the cleavage reaction significantly increases the steady-state catalytic rate of Rnt1p without affecting the initial burst phase Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information real-time analysis of Rnt1p cleavage kinetics, RNA binding to purified Rnt1p is monitored, Michaelis-Menten kinetics Saccharomyces cerevisiae
0.11
-
 RNA pH 8.5, 30°C, recombinant enzyme Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
KCl KCl reduces the stability of Rnt1p/RNA complex Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae C7GRB2
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-
Saccharomyces cerevisiae JAY291 C7GRB2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the catalytic efficiency of yeast ribonuclease III depends on substrate specific product release rate. Development of a real-time FRET assay for the detection of dsRNA degradation by yeast RNase III (Rnt1p) and detection of kinetic bottlenecks controlling the reactivity of different substrates. Rnt1p cleavage reaction is not only limited by the rate of catalysis but can also depend on base-pairing of product termini. Cleavage products terminating with paired nucleotides, like the degradation signals found in coding mRNA sequence, were less reactive and more prone to inhibition than products having unpaired nucleotides found in noncoding RNA substrates Saccharomyces cerevisiae ?
-
 ?
additional information the catalytic efficiency of yeast ribonuclease III depends on substrate specific product release rate. Development of a real-time FRET assay for the detection of dsRNA degradation by yeast RNase III (Rnt1p) and detection of kinetic bottlenecks controlling the reactivity of different substrates. Rnt1p cleavage reaction is not only limited by the rate of catalysis but can also depend on base-pairing of product termini. Cleavage products terminating with paired nucleotides, like the degradation signals found in coding mRNA sequence, were less reactive and more prone to inhibition than products having unpaired nucleotides found in noncoding RNA substrates Saccharomyces cerevisiae JAY291 ?
-
 ?
RNA + H2O RNA substrate tested are U5, U2, Mig2, and Yta6. Comparison between the association and dissociation kinetics of Mig2 and U5 products indicated that Mig2 products have a 2fold higher association rate and an 8fold lower dissociation rate. The reactivity of Rnt1p substrates is defined by the basepairing of the cleavage site, substrate specificity, overview Saccharomyces cerevisiae ?
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 ?
RNA + H2O RNA substrate tested are U5, U2, Mig2, and Yta6. Comparison between the association and dissociation kinetics of Mig2 and U5 products indicated that Mig2 products have a 2fold higher association rate and an 8fold lower dissociation rate. The reactivity of Rnt1p substrates is defined by the basepairing of the cleavage site, substrate specificity, overview Saccharomyces cerevisiae JAY291 ?
-
 ?

Synonyms

Synonyms Comment Organism
RNase III
-
 Saccharomyces cerevisiae
RNT1
-
 Saccharomyces cerevisiae
Rnt1p
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.08
-
 RNA pH 8.5, 30°C, recombinant enzyme Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Saccharomyces cerevisiae

General Information

General Information Comment Organism
evolution the enzyme is a member of the ribonuclease III (RNase III) family Saccharomyces cerevisiae
physiological function members of the ribonuclease III (RNase III) family regulate gene expression by triggering the degradation of double stranded RNA (dsRNA) Saccharomyces cerevisiae