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Literature summary for 3.1.26.3 extracted from

  • Carnes, J.; McDermott, S.; Stuart, K.
    RNase III domain of KREPB9 and KREPB10 association with editosomes in Trypanosoma brucei (2018), mSphere, 3, e00585-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.3

Cloned(Commentary)

Cloned (Comment) Organism
in procyclic-form parasite cells, V5-tagged KREPB9 or KREPB10 alleles are constitutively expressed from the tubulin locus in the background of the respective CN cell line in the absence of the tet-regulated expression of the wild-type allele in the rDNA locus Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
G238R site-directed mutagenesis of KREPB10, the mutant has reduced steady-state levels compared to wild-type KREPB10 Trypanosoma brucei
G238V site-directed mutagenesis of KREPB10, the mutant has reduced steady-state levels compared to wild-type KREPB10 Trypanosoma brucei
G270R site-directed mutagenesis of KREPB9, the G270R mutant protein is considerably weaker due to the lower steady-state level Trypanosoma brucei
G270V site-directed mutagenesis of KREPB9, mutant does not appear to shift compared to the wild-type Trypanosoma brucei
additional information generation of KREPB9 and KREPB10 null mutants. KREPB9 null cells show negligible differences in growth compared to parental 427 wild-type cells and single-knockout cells that retain KREPB9 expression. In KREPB10 null cells, growth is identical to single-knockout cells that retain KREPB10 expression, negligible differences from 427 wild-type cells Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
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 Trypanosoma brucei 5737
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mitochondrion
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 Trypanosoma brucei 5739
-

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei Q38F97
-
-
Trypanosoma brucei Q57X12
-
-
Trypanosoma brucei 927/4 GUTat10.1 Q38F97
-
-
Trypanosoma brucei 927/4 GUTat10.1 Q57X12
-
-

Synonyms

Synonyms Comment Organism
KREPB10
-
 Trypanosoma brucei
KREPB9
-
 Trypanosoma brucei
RNase III
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 Trypanosoma brucei
Tb09.160.3050
-
 Trypanosoma brucei
Tb927.8.5700
-
 Trypanosoma brucei

General Information

General Information Comment Organism
malfunction while RNA interference (RNAi) knockdown of either KREPB9 or KREPB10 produces no growth defect in procyclic-form parasites, the extent of the knockdown is relatively weak, with only 29% of KREPB9 mRNA or 43% of KREPB10 mRNA eliminated in each cell line Trypanosoma brucei
metabolism editosomes are the multiprotein complexes that catalyze the insertion and deletion of uridines to create translatable mRNAs in the mitochondria of kinetoplastids. Recognition and cleavage of a broad diversity of RNA substrates in vivo require three functionally distinct RNase III-type endonucleases (that act at distinct sites), as well as five additional editosome proteins that contain noncatalytic RNase III domains. RNase III domains have been identified in the editosome accessory proteins KREPB9 and KREPB10, suggesting a role related to editing endonuclease function, although KREPB9 and KREPB10 are not essential in either bloodstream-form parasites or procyclic-form parasites. Editosome interactions with KREPB9 and KREPB10 are mediated by the noncatalytic RNase III domain, consistent with a role in endonuclease specialization in Trypanosoma brucei. KREPB9 and KREPB10 are essential for editosome association, potentially via dimerization with RNase III domains in other editosome proteins Trypanosoma brucei
physiological function importance of RNase III domain interactions to editosome architecture. Association of KREPB9 and KREPB10 with about 20S editosomes requires the RNase III structure Trypanosoma brucei