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Literature summary for 3.1.26.3 extracted from

  • Gordon, G.; Cameron, J.; Pfleger, B.
    RNA sequencing identifies new RNase III cleavage sites in Escherichia coli and reveals increased regulation of mRNA (2017), mBio, 8, e00128-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.3

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information sequencing RNA from both an RNase III mutant (SK4455, rnc-14::DELTATn10 thyA715 rph-1) and the parental strain (MG1693, thyA715 rph-1) from which an RNase III mutant is derived Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ essentially required for activity Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A7Y0
-
-
Escherichia coli MG1693 P0A7Y0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information predicted secondary structure of substrates' RNase III sites, overview Escherichia coli ?
-
 ?
additional information predicted secondary structure of substrates' RNase III sites, overview Escherichia coli MG1693 ?
-
 ?

Synonyms

Synonyms Comment Organism
RNase III
-
 Escherichia coli
rnc
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli

General Information

General Information Comment Organism
malfunction pyruvate dehydrogenase activity is increased in an rnc deletion mutant compared to the wild-type strain in early stationary phase, confirming the link between RNA turnover and regulation of pathway activity Escherichia coli
metabolism influence of different RNase activities, including RNase III, on central metabolism, overview Escherichia coli
physiological function RNase III is a widespread endoribonuclease that binds and cleaves double-stranded RNA in many critical transcripts. RNase III cleavage at additional sites found in RNA of genes aceEF, proP, tnaC, dctA, pheM, sdhC, yhhQ, glpT, aceK, and gluQ accelerate RNA decay, consistent with previously described targets wherein RNase III cleavage initiates rapid degradation of secondary messages by other RNases. In contrast, cleavage at three sites in the ahpF, pflB, and yajQ transcripts lead to stabilized secondary transcripts. Two other sites in hisL and pheM overlap with transcriptional attenuators that likely serve to ensure turnover of these highly structured RNAs. Many of the additional RNase III target sites are located on transcripts encoding metabolic enzymes, while two RNase III sites are located within transcripts encoding enzymes near a key metabolic node connecting glycolysis and the tricarboxylic acid (TCA) cycle Escherichia coli