Cloned (Comment) | Organism |
---|---|
gene mc, DNA and amino acid sequence determination and analysis | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
D63A | site-directed mutagenesis, analysis of binding specificity and target sites compared to the wild-type enzyme | Staphylococcus aureus |
E135A | site-directed mutagenesis, analysis of binding specificity and target sites compared to the wild-type enzyme | Staphylococcus aureus |
additional information | effect of mutations in the catalytic site of Staphylococcus aureus RNase III, overview | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Staphylococcus aureus | enzyme RNase III cleavage produces RNA fragments with 5'-phosphate and 3'-hydroxyl termini and a two-nucleotide 3'-overhang. The 5' untranslated region of cspA mRNA is processed by the enzyme. Determination of substrate specificity by sequencing on cDNA libraries generated from RNAs that are co-immunoprecipitated with wild-type RNase III or two different cleavage-defective mutant variants D63A and E135A in vivo, validation of several RNA targets and mapping of cleavage sites of wild-type and mutant enzymes, detailed overview | ? | - |
? | |
additional information | Staphylococcus aureus RN6390 | enzyme RNase III cleavage produces RNA fragments with 5'-phosphate and 3'-hydroxyl termini and a two-nucleotide 3'-overhang. The 5' untranslated region of cspA mRNA is processed by the enzyme. Determination of substrate specificity by sequencing on cDNA libraries generated from RNAs that are co-immunoprecipitated with wild-type RNase III or two different cleavage-defective mutant variants D63A and E135A in vivo, validation of several RNA targets and mapping of cleavage sites of wild-type and mutant enzymes, detailed overview | ? | - |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
gene mc | - |
Staphylococcus aureus RN6390 | - |
gene mc | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme RNase III cleavage produces RNA fragments with 5'-phosphate and 3'-hydroxyl termini and a two-nucleotide 3'-overhang. The 5' untranslated region of cspA mRNA is processed by the enzyme. Determination of substrate specificity by sequencing on cDNA libraries generated from RNAs that are co-immunoprecipitated with wild-type RNase III or two different cleavage-defective mutant variants D63A and E135A in vivo, validation of several RNA targets and mapping of cleavage sites of wild-type and mutant enzymes, detailed overview | Staphylococcus aureus | ? | - |
? | |
additional information | enzyme RNase III cleavage produces RNA fragments with 5'-phosphate and 3'-hydroxyl termini and a two-nucleotide 3'-overhang. The 5' untranslated region of cspA mRNA is processed by the enzyme. Determination of substrate specificity by sequencing on cDNA libraries generated from RNAs that are co-immunoprecipitated with wild-type RNase III or two different cleavage-defective mutant variants D63A and E135A in vivo, validation of several RNA targets and mapping of cleavage sites of wild-type and mutant enzymes, detailed overview | Staphylococcus aureus RN6390 | ? | - |
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Synonyms | Comment | Organism |
---|---|---|
endoribonuclease III | - |
Staphylococcus aureus |
RNase III | - |
Staphylococcus aureus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme RNase III is a member of the ubiquitous family of double-strand-specific endoribonucleases | Staphylococcus aureus |
additional information | the enzyme autoregulates its own expression. Contributions of residues E135 and D63 to the active site of the enzyme | Staphylococcus aureus |
physiological function | the enzyme has a broad function in gene regulation in response to stress and during host infection of Staphylococcus aureus. RNase III-mediated cleavage in the 5' untranslated region enhances the stability and translation of cspA mRNA, which encodes the major cold-shock protein. Processing of cspA mRNA by the enzyme activates CspA synthesis. RNase III cleaves overlapping 5'-UTRs of divergently transcribed genes to generate leaderless mRNAs, which constitutes a distinct way to co-regulate neighboring genes. RNase III initiates maturation of rRNA operons. In addition to gene regulation, the enzyme is associated with RNA quality control of pervasive transcription, complexity of post-transcriptional regulation mediated by RNase III, possible function of the enzyme in the decay of structured regions of mRNAs, overview | Staphylococcus aureus |