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Literature summary for 3.1.26.3 extracted from

  • Lim, B.; Ahn, S.; Sim, M.; Lee, K.
    RNase III controls mltD mRNA degradation in Escherichia coli (2014), Curr. Microbiol., 68, 518-523.
    View publication on PubMed
Search for more literature for 3.1.26.3

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged RNase III in Escherichia coli Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information construction of strain MG1655 rnc-14::DELTATn10 from wild-type straiin HT115 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli RNase III is a double-stranded RNA-specific endoribonuclease that processes and degrades numerous mRNA molecules in Escherichia coli, it acts on mltD mRNA, which encodes membrane-bound lytic murein transglycosylase D. Introduction of a nucleotide substitution at the identified RNase III cleavage sites inhibited RNase III cleavage activity on mltD mRNA, resulting in, consequently, approximately two-fold increase in the steady-state level of the mRNA ?
-
 ?
additional information Escherichia coli HT115 RNase III is a double-stranded RNA-specific endoribonuclease that processes and degrades numerous mRNA molecules in Escherichia coli, it acts on mltD mRNA, which encodes membrane-bound lytic murein transglycosylase D. Introduction of a nucleotide substitution at the identified RNase III cleavage sites inhibited RNase III cleavage activity on mltD mRNA, resulting in, consequently, approximately two-fold increase in the steady-state level of the mRNA ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli HT115
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged RNase III from Escherichia coli by nickel affinity chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information RNase III is a double-stranded RNA-specific endoribonuclease that processes and degrades numerous mRNA molecules in Escherichia coli, it acts on mltD mRNA, which encodes membrane-bound lytic murein transglycosylase D. Introduction of a nucleotide substitution at the identified RNase III cleavage sites inhibited RNase III cleavage activity on mltD mRNA, resulting in, consequently, approximately two-fold increase in the steady-state level of the mRNA Escherichia coli ?
-
 ?
additional information RNase III is a double-stranded RNA-specific endoribonuclease that processes and degrades numerous mRNA molecules in Escherichia coli, it acts on mltD mRNA, which encodes membrane-bound lytic murein transglycosylase D. Introduction of a nucleotide substitution at the identified RNase III cleavage sites inhibited RNase III cleavage activity on mltD mRNA, resulting in, consequently, approximately two-fold increase in the steady-state level of the mRNA Escherichia coli HT115 ?
-
 ?

Synonyms

Synonyms Comment Organism
RNase III
-
 Escherichia coli

General Information

General Information Comment Organism
physiological function enzyme RNase III cleavage at A and B sites of mltD mRNA regulates mltD degradation, Escherichia coli