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Literature summary for 3.1.26.3 extracted from

  • Meng, W.; Nicholson, R.H.; Nathania, L.; Pertzev, A.V.; Nicholson, A.W.
    New approaches to understanding double-stranded RNA processing by ribonuclease III purification and assays of homodimeric and heterodimeric forms of RNase III from bacterial extremophiles and mesophiles (2008), Methods Enzymol., 447, 119-129.
    View publication on PubMed
Search for more literature for 3.1.26.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BLR(DE3) cells Escherichia coli
expression in Escherichia coli BL21(DE3)rnc105,recA Aquifex aeolicus
expression in Escherichia coli BL21(DE3)rnc105,recA Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Thermotoga maritima
Aquifex aeolicus RNase III also has been cocrystallized with dsRNA or specific hairpin substrates Aquifex aeolicus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus O67082
-
-
Escherichia coli
-
-
-
Thermotoga maritima Q9X0I6
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography. RNase III heterodimers also can be purified from the inclusion body Escherichia coli
Ni2+ affinity column Aquifex aeolicus
Ni2+ affinity column Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded RNA + H2O
-
 Escherichia coli 5'-phosphooligonucleotides
-
 ?
double-stranded RNA + H2O
-
 Aquifex aeolicus 5'-phosphooligonucleotides
-
 ?
double-stranded RNA + H2O
-
 Thermotoga maritima 5'-phosphooligonucleotides
-
 ?
additional information cleave internally 32P-labeled R1.1(WC) RNA Aquifex aeolicus ?
-
 ?
additional information cleaves internally 32P-labeled R1.1(WC) RNA Escherichia coli ?
-
 ?
additional information cleaves internally 32P-labeled R1.1(WC) RNA Thermotoga maritima ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer
-
 Aquifex aeolicus
heterodimer preparation of artificial heterodimers of RNase III, which are providing new insight on the subunit and domain interactions involved in dsRNA recognition and cleavage Escherichia coli

Synonyms

Synonyms Comment Organism
Aa-RNase III
-
 Aquifex aeolicus
ribonuclease III
-
 Escherichia coli
ribonuclease III
-
 Aquifex aeolicus
ribonuclease III
-
 Thermotoga maritima
RNase III
-
 Escherichia coli
RNase III
-
 Aquifex aeolicus
RNase III
-
 Thermotoga maritima
Tm-RNase III
-
 Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 hyperthermophilic bacterium Aquifex aeolicus
additional information
-
 hyperthermophilic bacterium Thermotoga maritima