Cloned (Comment) | Organism |
---|---|
into the pMAL-c2X vector, containing a TEV protease cleavage site at the EcoRI site, for expression in Escherichia coli Rosetta 2 cells | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Zea mays | 9507 | - |
chloroplast stroma | - |
Zea mays | 9570 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | A6YSL1 | - |
- |
Purification (Comment) | Organism |
---|---|
purified by affinity chromatography on amylose-coupled agarose resin, recombinant RNC1 binds RNA but lacks endonuclease activity | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Zea mays | - |
Storage Stability | Organism |
---|---|
-20°C, 40 mM Tris-HCl, pH 7.5, 300 mM NaCl, 1 mM EDTA, 5 mM 2-mercaptoethanol, 50% glycerol | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease III | - |
Zea mays |
RNase III | - |
Zea mays |
RNC1 | plant specific protein that contains two RNase III domains | Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
endonuclease assay | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
endonuclease assay | Zea mays |