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Literature summary for 3.1.26.3 extracted from

  • Conrad, C.; Schmitt, J.G.; Evguenieva-Hackenberg, E.; Klug, G.
    One functional subunit is sufficient for catalytic activity and substrate specificity of Escherichia coli endoribonuclease III artificial heterodimers (2002), FEBS Lett., 518, 93-96.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of a His-tagged E117Q mutant homodimer, a GST-tagged wild-type homodimer, and a His-tagged and GST-tagged wild-type/mutant heterodimer in strain BL21 Escherichia coli

Protein Variants

Protein Variants Comment Organism
E117Q mutant enzyme can still bind to the substrate RNA in presence of Mg2+ but cannot cleave it Escherichia coli
additional information construction of a heterodimer comprising one functional wild-type subunit and one inactive E117Q mutant subunit, which carries the E117Q mutation allowing the mutant subunit to bind but not cleave the substrate, the functional subunit is sufficient for catalytic activity of the heterodimer Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
KCl enzyme is sensitive to high salt conditions, above 50 mM, the mutant homodimer and the heterodimer are more sensitive than the wild-type Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25600
-
2 * 25600, wild-type enzyme, SDS-PAGE Escherichia coli
54000
-
recombinant His-tagged mutant homodimer, gel filtration Escherichia coli
78000
-
recombinant His-tagged and GST-tagged wild-type/mutant heterodimer, gel filtration Escherichia coli
103000
-
recombinant GST-tagged wild-type homodimer, gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant tagged heterodimer and homodimers from strain BL21 to homogeneity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dsRNA + H2O one functional monomer is sufficient for cleavage activity of the dimer Escherichia coli mature RNA
-
?

Subunits

Subunits Comment Organism
dimer 2 * 25600, wild-type enzyme, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
endoribonuclease III
-
Escherichia coli
RNase III
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli