Any feedback?
Literature summary for 3.1.26.3 extracted from
- Both N-terminal catalytic and C-terminal RNA binding domain contribute to substrate specificity and cleavage site selection of RNase III (2001), FEBS Lett., 509, 53-58.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of mutant hybrid proteins in Escherichia coli strain HMS174 | Escherichia coli |
| overexpression of mutant hybrid proteins in Escherichia coli strain HMS174 | Rhodobacter capsulatus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of hybrid proteins consisiting of the N-terminal nuclease domain of Rhodobacter capsulatus and the C-terminal dsRNA-binding domain of Escherichia coli and vice versa, extension of the spacer region between the N-terminal and C-terminal domains does not alter the cleavage specificity | Escherichia coli |
| additional information | construction of hybrid proteins consisting of the N-terminal nuclease domain of Rhodobacter capsulatus and the C-terminal dsRNA-binding domain of Escherichia coli and vice versa | Rhodobacter capsulatus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | - |
Escherichia coli |  |
| KCl | - |
Rhodobacter capsulatus | |
| Mg2+ | activity with recombinant hybrid enzyme mutants, overview | Escherichia coli | |
| Mg2+ | activity with recombinant hybrid enzyme mutants, overview | Rhodobacter capsulatus | |
| Mn2+ | activity with recombinant hybrid enzyme mutants, overview | Escherichia coli | |
| Mn2+ | activity with recombinant hybrid enzyme mutants, overview | Rhodobacter capsulatus | |
| additional information | metal ion specificity of the enzyme is determined by the N-terminus | Escherichia coli | |
| additional information | metal ion specificity of the enzyme is determined by the N-terminus | Rhodobacter capsulatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ds-rRNA + H2O | Escherichia coli | - |
mature ds-rRNA | - |
? | |
| ds-rRNA + H2O | Rhodobacter capsulatus | - |
mature ds-rRNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene rnc, strain JM109, recombinant hybrid enzymes consisiting of the N-terminal nuclease domain of Rhodobacter capsulatus and the C-terminal dsRNA-binding domain of Escherichia coli, and vice versa | - |
| Rhodobacter capsulatus | - |
gene rnc, strain 37b4, recombinant hybrid enzymes consisiting of the N-terminal nuclease domain of Rhodobacter capsulatus and the C-terminal dsRNA-binding domain of Escherichia coli, and vice versa | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant hybrid proteins to homogeneity | Escherichia coli |
| recombinant hybrid proteins to homogeneity | Rhodobacter capsulatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ds-rRNA + H2O | - |
Escherichia coli | mature ds-rRNA | - |
? | |
| ds-rRNA + H2O | - |
Rhodobacter capsulatus | mature ds-rRNA | - |
? | |
| ds-rRNA + H2O | double-strand RNA specific endonuclease | Escherichia coli | mature ds-rRNA | - |
? | |
| dsRNA + H2O | double-strand RNA specific endonuclease | Rhodobacter capsulatus | mature RNA | - |
? | |
| additional information | substrate specificity of the recombinant hybrid enzyme mutants, substrate specificity is determined by the catalytic N-terminus of the enzyme | Escherichia coli | ? | - |
? | |
| additional information | substrate specificity of the recombinant hybrid enzyme mutants, substrate specificity is determined by the catalytic N-terminus of the enzyme | Rhodobacter capsulatus | ? | - |
? | |
| R1.1 RNA + H2O | based on the R1.1 processing signal, which is encoded in the phage T7 genetic early region between genes 1.0 and 1.1, recombinant substrate from in vitro transcription, determination of cleaving positions for the recombinant hybrid enzyme mutants | Escherichia coli | fragments of R1.1 RNA | - |
? | |
| R1.1 RNA + H2O | based on the R1.1 processing signal, which is encoded in the phage T7 genetic early region between genes 1.0 and 1.1, recombinant substrate from in vitro transcription, determination of cleaving positions for the recombinant hybrid enzyme mutants | Rhodobacter capsulatus | fragments of R1.1 RNA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase III | - |
Escherichia coli |
| RNase III | - |
Rhodobacter capsulatus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
