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Literature summary for 3.1.26.3 extracted from

  • Conrad, C.; Rauhut, R.; Klug, G.
    Different cleavage specificities of RNases III from Rhodobacter capsulatus and Escherichia coli (1998), Nucleic Acids Res., 26, 4446-4453.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.3

Activating Compound

Activating Compound Comment Organism Structure
NH4+
-
 Escherichia coli
NH4+
-
 Rhodobacter capsulatus

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the rnc gene in Escherichia coli Rhodobacter capsulatus

Inhibitors

Inhibitors Comment Organism Structure
K+
-
 Escherichia coli
K+ inhibits at concentrations higher than 200 mM Rhodobacter capsulatus
NH4+
-
 Escherichia coli
NH4+ inhibits at concentrations higher than 200 mM Rhodobacter capsulatus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ stimulates Escherichia coli
Co2+ stimulates Rhodobacter capsulatus
K+
-
 Escherichia coli
K+
-
 Rhodobacter capsulatus
Mg2+ stimulates Escherichia coli
Mg2+ stimulates Rhodobacter capsulatus
Mn2+ stimulates Escherichia coli
Mn2+ stimulates Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded RNA + H2O Escherichia coli cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA 5'-phosphooligonucleotides
-
 ?
double-stranded RNA + H2O Rhodobacter capsulatus cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA 5'-phosphooligonucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Rhodobacter capsulatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
of the native and recombinant protein Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded RNA + H2O bacteriophage T7 RNA Escherichia coli 5'-phosphooligonucleotides
-
 ?
double-stranded RNA + H2O bacteriophage T7 RNA Rhodobacter capsulatus 5'-phosphooligonucleotides
-
 ?
double-stranded RNA + H2O cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA Escherichia coli 5'-phosphooligonucleotides
-
 ?
double-stranded RNA + H2O cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA Rhodobacter capsulatus 5'-phosphooligonucleotides
-
 ?
ribosomal RNA + H2O 23S RNA in Rhodobacter capsulatus Escherichia coli smaller precursor rRNA
-
 ?
ribosomal RNA + H2O 23S RNA in Rhodobacter capsulatus Rhodobacter capsulatus smaller precursor rRNA
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
 Rhodobacter capsulatus