Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.26.13 extracted from

  • Goedken, E.R.; Marqusee, S.
    Metal binding and activation of the ribonuclease H domain from Moloney murine leukemia virus (1999), Protein Eng., 12, 975-980.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D524N less than 0.5% of wild-type activity, no binding of Mn2+ Moloney murine leukemia virus
D583N less than 0.5% of wild-type activity, no binding of Mn2+ Moloney murine leukemia virus
D653N 4% of wild-type activity Moloney murine leukemia virus
E562Q less than 0.5% of wild-type activity, no binding of Mn2+ Moloney murine leukemia virus
H638G 15% of wild-type activity Moloney murine leukemia virus

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ activation at 0.01-1 mM, inhibitory above Moloney murine leukemia virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mn2+ is preferred over Mg2+. One-metal catalytic mechanism for the Mn2+/Mg2+-dependent activities Moloney murine leukemia virus
Mn2+ activation at 0.01-1 mM, inhibitory above. Mn2+ is preferred over Mg2+. One-metal catalytic mechanism for the Mn2+/Mg2+-dependent activities Moloney murine leukemia virus

Organism

Organism UniProt Comment Textmining
Moloney murine leukemia virus
-
-
-

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
29.7
-
melting temperature, wild-type Moloney murine leukemia virus
29.7
-
melting temperature, mutant H638G Moloney murine leukemia virus
29.9
-
melting temperature, wild-type, presence of 25 mM NaCl Moloney murine leukemia virus
34.1
-
melting temperature, wild-type, presence of 1 mM Mg2+ Moloney murine leukemia virus
35.8
-
melting temperature, mutant E562Q Moloney murine leukemia virus
39.2
-
melting temperature, mutant D653N Moloney murine leukemia virus
42
-
melting temperature, wild-type, presence of 1 mM Mn2+ Moloney murine leukemia virus
43.5
-
melting temperature, mutant H638G, presence of 1 mM Mn2+ Moloney murine leukemia virus
45.8
-
melting temperature, mutant D524N Moloney murine leukemia virus
46.3
-
melting temperature, mutant D524N, presence of 1 mM Mn2+ Moloney murine leukemia virus
46.9
-
melting temperature, mutant D653N, presence of 1 mM Mn2+ Moloney murine leukemia virus
48.4
-
melting temperature, mutant D583N, presence of 1 mM Mn2+ Moloney murine leukemia virus
48.8
-
melting temperature, mutant E562Q, presence of 1 mM Mn2+ Moloney murine leukemia virus
48.9
-
melting temperature, mutant D583N Moloney murine leukemia virus