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Literature summary for 3.1.26.13 extracted from

  • Ghosh, M.; Williams, J.; Powell, M.D.; Levin, J.G.; Le Grice, S.F.
    Mutating a conserved motif of the HIV-1 reverse transcriptase palm subdomain alters primer utilization (1997), Biochemistry, 36, 5758-5768.
    View publication on PubMed
Search for more literature for 3.1.26.13

Protein Variants

Protein Variants Comment Organism
W229F mutation in primer grip residue, specificity of cleavage is not compromised, efficiency is reduced to 33-44% of wild-type Human immunodeficiency virus 1
W229F/Y232W mutation in primer grip residues, specificity of cleavage is not compromised, efficiency is reduced to 33-44% of wild-type Human immunodeficiency virus 1
W229Y mutation in primer grip residue, specificity of cleavage is not compromised, efficiency is reduced to 33-44% of wild-type Human immunodeficiency virus 1
Y229F/Y232F mutation in primer grip residues, specificity of cleavage is not compromised, efficiency is reduced to 33-44% of wild-type Human immunodeficiency virus 1
Y232W mutation in primer grip residue, specificity of cleavage is not compromised, efficiency is reduced to 33-44% of wild-type Human immunodeficiency virus 1

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate heteropolymeric 90-nt 5' end-labeled RNA template-annealed to a 36-nt DNA primer is cleaved by wild-type p66/p51 RT precisely at the RNA/DNA junction to liberate a 20-nt (+) strand DNA Human immunodeficiency virus 1 ?
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