Protein Variants | Comment | Organism |
---|---|---|
additional information | neither the native nor N-terminal extended form of RNase G can restore the growth defect associated with either the rne-1 or rneD1018 alleles even when expressed at very high protein levels. In contrast, two distinct spontaneously derived single amino acid substitutions within the predicted RNase H domain of RNase G, generating the rng-219 and rng-248 alleles, result in complementation of the growth defect associated with various RNase E mutants. Construction of rneD1018/rng-219 and rneD1018/rng-248 double mutants. Domain swaps between RNase E and RNase G generate proteins that do not complement RNase E deficiency | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNACys + H2O | Escherichia coli | - |
tRNACys + 3'-leader of tRNA | - |
? | |
pre-tRNAHis + H2O | Escherichia coli | - |
tRNAHis + 3'-leader of tRNA | - |
? | |
pre-tRNAPro + H2O | Escherichia coli | - |
tRNAPro + 3'-leader of tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | - |
gene rne | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNACys + H2O | - |
Escherichia coli | tRNACys + 3'-leader of tRNA | - |
? | |
pre-tRNAHis + H2O | - |
Escherichia coli | tRNAHis + 3'-leader of tRNA | - |
? | |
pre-tRNAPro + H2O | - |
Escherichia coli | tRNAPro + 3'-leader of tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endoribonuclease E | - |
Escherichia coli |
endoribonuclease RNase E | - |
Escherichia coli |
RNase E | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | absence of RNase E differentially affects the decay of specific mRNAs. Neither the native nor N-terminal extended form of RNase G can restore the growth defect associated with either the rne-1 or rneD1018 alleles even when expressed at very high protein levels. In contrast, two distinct spontaneously derived single amino acid substitutions within the predicted RNase H domain of RNase G, generating the rng-219 and rng-248 alleles, result in complementation of the growth defect associated with various RNase E mutants | Escherichia coli |
metabolism | the endoribonuclease RNase E of Escherichia coli is an essential enzyme that plays a major role in all aspects of RNA metabolism | Escherichia coli |
physiological function | RNase E is essential for cell viability and plays a major role in mRNA decay, rRNA maturation, tRNA processing, and a variety of other aspects of RNA metabolism. Maturation of tRNACys, tRNAHis, and tRNAPro but not tRNAAsn is completely dependent on RNase E | Escherichia coli |
physiological function | neither the native nor N-terminal extended form of RNase G can restore the growth defect associated with RNase E deletion mutants even when expressed at very high protein levels. In contrast, two distinct spontaneously derived single amino acid substitutions within the predicted RNase H domain of RNase G, generating the rng-219 and rng-248 alleles, result in complementation of the growth defect associated with various RNase E mutants, suggesting that this region of the two proteins may help distinguish their in vivo biological activities | Escherichia coli |