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Literature summary for 3.1.26.12 extracted from

  • Stazic, D.; Lindell, D.; Steglich, C.
    Antisense RNA protects mRNA from RNase E degradation by RNA-RNA duplex formation during phage infection (2011), Nucleic Acids Res., 39, 4890-4899.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.12

Application

Application Comment Organism
medicine extraordinarily long antisense RNAs of 3.5 and 7 kb protect a set of mRNAs from RNase E degradation that accumulate during phage infection. These antisense RNA-mRNA duplex formations mask single-stranded recognition sites of RNase E, leading to increased stability of the mRNAs. The interactions directly modulate RNA stability and provide an explanation for enhanced transcript abundance of certain mRNAs during phage infection Prochlorococcus sp.

Cloned(Commentary)

Cloned (Comment) Organism
recombinant overexpression of the His-tagged enzyme in Escherichia coli Prochlorococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimum concentration 12 mM Prochlorococcus sp.

Organism

Organism UniProt Comment Textmining
Prochlorococcus sp.
-
-
-
Prochlorococcus sp. MED4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Prochlorococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information fluorogenic cleavage assay Prochlorococcus sp. ?
-
 ?
additional information fluorogenic cleavage assay Prochlorococcus sp. MED4 ?
-
 ?

Synonyms

Synonyms Comment Organism
RNase E
-
 Prochlorococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Prochlorococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Prochlorococcus sp.

General Information

General Information Comment Organism
physiological function extraordinarily long antisense RNAs of 3.5 and 7 kb protect a set of mRNAs from RNase E degradation that accumulate during phage infection. These antisense RNA-mRNA duplex formations mask single-stranded recognition sites of RNase E, leading to increased stability of the mRNAs. The interactions directly modulate RNA stability and provide an explanation for enhanced transcript abundance of certain mRNAs during phage infection Prochlorococcus sp.