Protein Variants | Comment | Organism |
---|---|---|
A327P | site-directed mutagensis, temperature-sensitive mutant | Salmonella enterica |
A327P | mutation in N-terminal part of enzyme, temperature-sensitive mutation that is able to suppress the slow growth caused by the mutation tufA499 at permissive temperatures. In addition, mutation causes a large increase 503 in rne mRNA steady state levels | Salmonella enterica |
A448V | site-directed mutagensis, the mutation causes steric problemes and leads to conformational changes | Salmonella enterica |
C471Y | site-directed mutagensis, the mutation causes steric problemes and leads to conformational changes | Salmonella enterica |
G113D | site-directed mutagensis, the mutation causes steric problemes and leads to conformational changes | Salmonella enterica |
G66C | site-directed mutagensis, temperature-sensitive mutant, the mutation causes steric problemes and leads to conformational changes | Salmonella enterica |
G66C | mutation in N-terminal part of enzyme, temperature-sensitive mutation that is able to suppress the slow growth caused by the mutation tufA499 at permissive temperatures. In addition, mutation causes a large increase 503 in rne mRNA steady state levels | Salmonella enterica |
I207N | site-directed mutagensis, temperature-sensitive mutant, the mutation reduces the nonpolar contacts in the core, which may lead to a less stable protein | Salmonella enterica |
I207N | mutation in N-terminal part of enzyme, temperature-sensitive mutation that is able to suppress the slow growth caused by the mutation tufA499 at permissive temperatures. In addition, mutation causes a large increase 503 in rne mRNA steady state levels | Salmonella enterica |
I207S | site-directed mutagensis, temperature-sensitive mutant, the mutation reduces the nonpolar contacts in the core, which may lead to a less stable protein | Salmonella enterica |
I207S | mutation in N-terminal part of enzyme, temperature-sensitive mutation that is able to suppress the slow growth caused by the mutation tufA499 at permissive temperatures. In addition, mutation causes a large increase 503 in rne mRNA steady state levels | Salmonella enterica |
L424R | site-directed mutagensis, the mutation reduces the nonpolar contacts in the core, which may lead to a less stable protein | Salmonella enterica |
additional information | identification of the EF-Tu mutation, tufA499, with a slow-groth phenotype, structural basis of properties of suppressors of the mutation, overview. Isolation and identification of temperature-sensitive mutations in RNase E that suppress the slow growth of tufA499. Mutations in rne affect the steady-state level of RNase E mRNA. The rne ts and ts suppressor mutations affect 9S to 5S rRNA processing, growrh profiles, overview. The ts mutations in RNase E affect the maturation of hisR tRNAHis | Salmonella enterica |
V459G | site-directed mutagensis, the mutation reduces the nonpolar contacts in the core, which may lead to a less stable protein | Salmonella enterica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | - |
- |
- |
Salmonella enterica | - |
serovar typhimurium | - |
Synonyms | Comment | Organism |
---|---|---|
RNase E | - |
Salmonella enterica |
General Information | Comment | Organism |
---|---|---|
physiological function | RNase E is a key component of the RNA degradosome, a multienzyme complex hat plays a central role in mRNA turnover and the processing of stable RNA in eubacteria. The degradosome is composed of a tetramer of RNase E molecules interacting via their N-terminal regions, with the C-terminal end of each RNase E molecule complexed with a monomer of RhlB, a dimer of enolase, and a trimer of PNPase | Salmonella enterica |