Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
N305D | the mutation is localized in the catalytic domain of RNase E | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | localized to the inner cytoplasmic membrane, segment-A is necessary and sufficient for RNase E binding to membranes | Escherichia coli | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
464000 | - |
co-sedimentation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli KSL2000 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
RNase E is renatured on the Ni-NTA resin by decreasing the concentration of urea to 0.8 M | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
9SA RNA + H2O | 9Sa is a fragment of the 9S precursor of 5S rRNA | Escherichia coli | ? | - |
? | |
9SA RNA + H2O | 9Sa is a fragment of the 9S precursor of 5S rRNA | Escherichia coli KSL2000 | ? | - |
? | |
RNA + H2O | - |
Escherichia coli | ? | - |
? | |
RNA + H2O | - |
Escherichia coli KSL2000 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | co-sedimentation | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
RNase E | RNase E is an essential endoribonuclease involved in RNA processing and mRNA degradation | Escherichia coli |