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Literature summary for 3.1.26.12 extracted from

  • Kime, L.; Jourdan, S.S.; McDowall, K.J.
    Identifying and characterizing substrates of the RNase E/G family of enzymes (2008), Methods Enzymol., 447, 215-241.
    View publication on PubMed
Search for more literature for 3.1.26.12

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00207
-
 5'-GGGACAGUAUUUG-3' in 25 mM bis-Tris-propane (pH 8.3), 15 mM MgCl2, 100 mM NaCl, 0.1% (v/v) Triton X-100, and 1 mM dithiothreitol Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal affinity chromatography and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-GGGACAGUAUUUG-3' + H2O
-
 Escherichia coli ?
-
 ?
9S precursor RNA + H2O
-
 Escherichia coli p5S RNA + ?
-
 ?

Synonyms

Synonyms Comment Organism
RNase E
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4
-
 5'-GGGACAGUAUUUG-3' in 25 mM bis-Tris-propane (pH 8.3), 15 mM MgCl2, 100 mM NaCl, 0.1% (v/v) Triton X-100, and 1 mM dithiothreitol Escherichia coli