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Literature summary for 3.1.26.12 extracted from

  • Mackie, G.A.; Coburn, G.A.; Miao, X.; Briant, D.J.; Prudhomme-Genereux, A.; Stickney, L.M.; Hankins, J.S.
    Preparation of the Escherichia coli RNase E protein and reconstitution of the RNA degradosome (2008), Methods Enzymol., 447, 199-213.
    View publication on PubMed
Search for more literature for 3.1.26.12

Organism

Organism UniProt Comment Textmining
Escherichia coli
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Escherichia coli GM402 BL21(DE3)
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-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Toyopearl column filtration chromatography, and Affi-Gel blue column chromatography Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
the diluted Rne is dialyzed against two changes of 100 volumes each of 60 mM NH4Cl, 0.1 mM dithiothreitol, and 10% (v/v) glycerol at 4°C for at least 20 h Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the C-terminus of this enzyme serves as a scaffold to which other components of the RNA degradosome bind including the phosphorolytic 3'-exonuclease, polynucleotide phosphorylase, the DEAD-box RNA helicase RhlB, and the glycolytic enzyme enolase. The DEAD-box RNA helicases CsdA and RhlE and the RNA binding protein Hfq may bind to RNase E in place of one or more of the prototypical components. Escherichia coli ?
-
 ?
additional information the C-terminus of this enzyme serves as a scaffold to which other components of the RNA degradosome bind including the phosphorolytic 3'-exonuclease, polynucleotide phosphorylase, the DEAD-box RNA helicase RhlB, and the glycolytic enzyme enolase. The DEAD-box RNA helicases CsdA and RhlE and the RNA binding protein Hfq may bind to RNase E in place of one or more of the prototypical components. Escherichia coli GM402 BL21(DE3) ?
-
 ?
RNA + H2O
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 Escherichia coli ?
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 ?
RNA + H2O
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 Escherichia coli GM402 BL21(DE3) ?
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 ?

Synonyms

Synonyms Comment Organism
RNase E
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 Escherichia coli
Rne protein
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 Escherichia coli