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Literature summary for 3.1.26.12 extracted from

  • Feng, Y.; Vickers, T.A.; Cohen, S.N.
    The catalytic domain of RNase E shows inherent 3' to 5' directionality in cleavage site selection (2002), Proc. Natl. Acad. Sci. USA, 99, 14746-14751.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information 5' monophosphorylation increases the enzyme activity via altered binding of the catalytic domain N-Rne Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
gene rne, overexpression in strain BL21(DE3) as His-tagged enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
additional information 2'-O-methyl nucleotide substitutions in the synthetic RNA substrate, e.g. in RNA BR13-13M, inhibit the enzyme Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Escherichia coli
NaCl present in enzyme assay at 10 mM Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
gene rne
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from strain BL21(DE3) Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
BR13 RNA + H2O synthetic RNA substrate, the cleavage site is GGGACAGUAUUUG Escherichia coli ?
-
?
BR13N RNA + H2O synthetic RNA substrate, the cleavage site is GGGACAGUCUGUG Escherichia coli ?
-
?
additional information the catalytic domain of the multifunctional endoribonuclease determines inherent 3' to 5' directionality in cleavage site selection, cleavage site sequences overview Escherichia coli ?
-
?
RNA I + H2O
-
Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
More the N-terminal region N-Rne contains the catalytic domain Escherichia coli

Synonyms

Synonyms Comment Organism
RNase E
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Escherichia coli