Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | 5' monophosphorylation increases the enzyme activity via altered binding of the catalytic domain N-Rne | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
gene rne, overexpression in strain BL21(DE3) as His-tagged enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | 2'-O-methyl nucleotide substitutions in the synthetic RNA substrate, e.g. in RNA BR13-13M, inhibit the enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli | |
NaCl | present in enzyme assay at 10 mM | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene rne | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from strain BL21(DE3) | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
BR13 RNA + H2O | synthetic RNA substrate, the cleavage site is GGGACAGUAUUUG | Escherichia coli | ? | - |
? | |
BR13N RNA + H2O | synthetic RNA substrate, the cleavage site is GGGACAGUCUGUG | Escherichia coli | ? | - |
? | |
additional information | the catalytic domain of the multifunctional endoribonuclease determines inherent 3' to 5' directionality in cleavage site selection, cleavage site sequences overview | Escherichia coli | ? | - |
? | |
RNA I + H2O | - |
Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the N-terminal region N-Rne contains the catalytic domain | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
RNase E | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |