Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | anti-sense deoxynucleotide constructs complementary to the 5' end sequences of RNA substrates reduce the enzyme activity | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the enzyme is localized in a multi-protein complex, the RNA degradosome | Escherichia coli | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
9S rRNA precursor + H2O | Escherichia coli | - |
5S rRNA + ? | - |
? | |
9S rRNA precursor + H2O | Escherichia coli CF 881 | - |
5S rRNA + ? | - |
? | |
rpsT mRNA + H2O | Escherichia coli | the substrate encodes the ribosomal protein S20 | ? | - |
? | |
rpsT mRNA + H2O | Escherichia coli CF 881 | the substrate encodes the ribosomal protein S20 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene rne | - |
Escherichia coli CF 881 | - |
gene rne | - |
Purification (Comment) | Organism |
---|---|
native degradosomes from strain CF881, which lacks RNase I, further electrophoretically purification of Rne protein | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
9S rRNA precursor + H2O | - |
Escherichia coli | 5S rRNA + ? | - |
? | |
9S rRNA precursor + H2O | very low activity with a covalently closed circular variant of the substrate compared to the linear one, overview | Escherichia coli | 5S rRNA + ? | - |
? | |
9S rRNA precursor + H2O | - |
Escherichia coli CF 881 | 5S rRNA + ? | - |
? | |
9S rRNA precursor + H2O | very low activity with a covalently closed circular variant of the substrate compared to the linear one, overview | Escherichia coli CF 881 | 5S rRNA + ? | - |
? | |
additional information | ribonuclease E is a 5'-end-dependent, single-strand-specific endonuclease that initiates the selective decay of mRNA having regulatory function, regulation of mRNA levels in response to environment, the enzyme proceeds in a 5' to 3' direction, the enzyme shows high activity with 5' monophosphorylated RNA, but low activity with 5' triphosphorylated RNA, poor activity with circular RNA substrates, overview | Escherichia coli | ? | - |
? | |
additional information | ribonuclease E is a 5'-end-dependent, single-strand-specific endonuclease that initiates the selective decay of mRNA having regulatory function, regulation of mRNA levels in response to environment, the enzyme proceeds in a 5' to 3' direction, the enzyme shows high activity with 5' monophosphorylated RNA, but low activity with 5' triphosphorylated RNA, poor activity with circular RNA substrates, overview | Escherichia coli CF 881 | ? | - |
? | |
rpsT mRNA + H2O | the substrate encodes the ribosomal protein S20 | Escherichia coli | ? | - |
? | |
rpsT mRNA + H2O | the substrate encodes the ribosomal protein S20, very low activity with a covalently closed circular variant of the substrate compared to the linear one, overview | Escherichia coli | ? | - |
? | |
rpsT mRNA + H2O | the substrate encodes the ribosomal protein S20 | Escherichia coli CF 881 | ? | - |
? | |
rpsT mRNA + H2O | the substrate encodes the ribosomal protein S20, very low activity with a covalently closed circular variant of the substrate compared to the linear one, overview | Escherichia coli CF 881 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme is localized in a multi-protein complex, the RNA degradosome | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease E | - |
Escherichia coli |
RNase E | - |
Escherichia coli |
Rne protein | - |
Escherichia coli |