Cloned (Comment) | Organism |
---|---|
expression in wild-type rne cells and in thermosensitive mutant rne-50 and rne-3071 cells, overexpression of truncated 110 kDa mutant enzyme in rne50 cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutation of gene rne affect the rate of mRNA decay in vivo, construction of a truncated 110 kDa mutant enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | enzyme assay in presence of 1 mM MnCl2 | Escherichia coli | |
NH4Cl | enzyme assay in presence of 0.1 M NH4Cl | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | - |
x * 180000, unprocessed active enzyme, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ompA mRNA + H2O | Escherichia coli | ompA at a site which is rate determining for degradation and also cleaved by RNase K | ? | - |
? | |
rne mRNA + H2O | Escherichia coli | the enzyme autoregulates its expression by cleavage and processing of its own rne mRNA | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene rne or ams | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the recombinant enzyme is cleaved from an active 180 kDa form to active about 70 kDa and 60 kDa fragments during purification | Escherichia coli |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme and truncated mutant enzyme, partially | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme autoregulates its expression by cleavage and processing of its own rne mRNA | Escherichia coli | ? | - |
? | |
ompA mRNA + H2O | processing | Escherichia coli | ? | - |
? | |
ompA mRNA + H2O | ompA at a site which is rate determining for degradation and also cleaved by RNase K | Escherichia coli | ? | - |
? | |
rne mRNA + H2O | the enzyme autoregulates its expression by cleavage and processing of its own rne mRNA | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 180000, unprocessed active enzyme, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
endoribonuclease RNase E | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |