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Literature summary for 3.1.26.12 extracted from

  • Jiang, X.; Diwa, A.; Belasco, J.G.
    Regions of RNase E important for 5'-end-dependent RNA cleavage and autoregulated synthesis (2000), J. Bacteriol., 182, 2468-2475.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.12

Activating Compound

Activating Compound Comment Organism Structure
additional information a monophosphate at the 5' end of the RNA substrate stimulates the enzyme Escherichia coli K-12

Cloned(Commentary)

Cloned (Comment) Organism
gene rne, overexpression in Escherichia coli, functional complementation of the RNase E-deficient strain CJ1832 of Escherichia coli Synechocystis sp.
overexpression of active His- and Myc-tagged truncated enzyme in strain BL21(DE3) Escherichia coli K-12

Protein Variants

Protein Variants Comment Organism
additional information construction of the truncated mutant enzyme N-RNase E consisting of the N-terminal catalytic site, residues 1-498, an enzyme-deficient strain CJ1832 can be complemented by expression of SynRne of Synechocystis sp., but not by Escherichia coli CafA, i.e. RNase G Escherichia coli K-12

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Synechocystis sp.
Mg2+
-
 Escherichia coli K-12
NH4+ enzyme assay in presence of 0.1 M NH4Cl Synechocystis sp.
NH4+ enzyme assay in presence of 0.1 M NH4Cl Escherichia coli K-12

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RNA + H2O Escherichia coli K-12 the enzyme plays a key role in processing and degradation of RNA in Escherichia coli ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli K-12
-
 strains CJ1827 and CJ1828 derived from strain MC1061, gene rne or ams
-
Synechocystis sp.
-
 strain PCC 6803, gene rne
-

Purification (Commentary)

Purification (Comment) Organism
recombinant active His- and Myc-tagged truncated enzyme from strain BL21(DE3) by affinity chromatography Escherichia coli K-12
recombinant enzyme from Escherichia coli Synechocystis sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 relative activity of different strains, comparison of wild-type RNase E and mutant N-RNase E to SynRne of Synechocystis sp., overview Escherichia coli K-12
additional information
-
 relative activity of SynRne expressed in Escherichia coli compared to the Escherichia coli enzyme, overview Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-capped RNA I.26 + H2O low activity, cleavage of the 5' substrate end Escherichia coli K-12 ?
-
 ?
9S rRNA + H2O
-
 Synechocystis sp. ?
-
 ?
pppRNA I.26 + H2O low activity, cleavage of the 5' substrate end Escherichia coli K-12 ?
-
 ?
pRNA I.26 + H2O a monophosphate at the 5' end of the RNA I substrate stimulates the enzyme 25-30fold, cleavage of the 5' substrate end Escherichia coli K-12 ?
-
 ?
RNA + H2O the enzyme plays a key role in processing and degradation of RNA in Escherichia coli Escherichia coli K-12 ?
-
 ?
RNA I + H2O
-
 Synechocystis sp. ?
-
 ?
RNA I + H2O cleavage of the 5' substrate end Escherichia coli K-12 ?
-
 ?
RNA I.26 + H2O
-
 Synechocystis sp. ?
-
 ?
RNA I.26 + H2O cleavage of the 5' substrate end Escherichia coli K-12 ?
-
 ?

Subunits

Subunits Comment Organism
More the catalytic site is located at the N-terminus, residues 1-406 Synechocystis sp.
More the catalytic site is located at the N-terminus, residues 1-413 Escherichia coli K-12

Synonyms

Synonyms Comment Organism
RNase E
-
 Synechocystis sp.
RNase E
-
 Escherichia coli K-12
SynRne
-
 Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Synechocystis sp.
30
-
 assay at Escherichia coli K-12

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Synechocystis sp.
7.5
-
 assay at Escherichia coli K-12