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Literature summary for 3.1.26.12 extracted from

  • Kaberdin, V.R.; Bizebard, T.
    Characterization of Aquifex aeolicus RNase E/G (2005), Biochem. Biophys. Res. Commun., 327, 382-392.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene rng, overexpression of His10-tagged RNase E/G, Rng, in Escherichia coli strain BL21(DE3) Aquifex aeolicus
overexpression of His10-tagged enzyme in Escherichia coli strain BL21(DE3) Aquifex aeolicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Aquifex aeolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Aquifex aeolicus Aquifex aeolicus RNase E/G is able to selectively cleave internucleotide bonds in the 3'-5' direction, and to cut in intercistronic regions of putative tRNA precursors ?
-
?
RNA + H2O Aquifex aeolicus the enzyme is required for RNA processing and degradation ?
-
?

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
gene rng
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His10-tagged enzyme from Escherichia coli strain BL21(DE3) by His-trap chromatography with or without cleavage of the His-tag by factor Xa Aquifex aeolicus
recombinant His10-tagged RNase E/G from Escherichia coli strain BL21(DE3) by metal affinity chromatography, removal of the His-tag by factorXa and dialysis Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
9SA RNA + H2O
-
Aquifex aeolicus ?
-
?
BR13 RNA + H2O i.e. 5'GGGACAGUAUUUG3', 3' fluorescein-labeled substrate Aquifex aeolicus ?
-
?
additional information the enzyme prefers 5' monophosphorylated RNA substrates compared to nonphosphorylated or 5' triphosphorylated RNA substrates Aquifex aeolicus ?
-
?
additional information Aquifex aeolicus RNase E/G is able to selectively cleave internucleotide bonds in the 3'-5' direction, and to cut in intercistronic regions of putative tRNA precursors Aquifex aeolicus ?
-
?
additional information site-specific RNase E/G cleavage of RNA using 5'-end-labelled substrates, e.g. L1 RNA, RNAI, and 9S RNA, overview. RNase E/G has a temperature-dependent, endoribonucleolytic activity that is dependent on the 5'-phosphorylation status of RNA. The enzyme site-specifically cleaves oligonucleotides and structured RNAs at locations that are partly overlapping or completely different when compared to the positions of Escherichia coli RNase E and RNase G cleavage sites, RNase E/G shows 3'-5' directionality in cleavage site selection, overview, the cleavage site selection of RNase E/G is temperature-dependent, overview Aquifex aeolicus ?
-
?
Omp11 RNA + H2O
-
Aquifex aeolicus ?
-
?
RNA + H2O the enzyme is required for RNA processing and degradation Aquifex aeolicus ?
-
?
RNA + H2O cleavage site specificity is not affected by temperature, selective cleavage at the 5' end of internucleotide bonds in 3' to 5' direction, cleavage pattern, overview Aquifex aeolicus ?
-
?
RNA I + H2O
-
Aquifex aeolicus ?
-
?
tRNA precursors + H2O the enzyme, cuts intercistronic regions of putative tRNA precursors, overview Aquifex aeolicus ?
-
?

Synonyms

Synonyms Comment Organism
AqaRng
-
Aquifex aeolicus
RNase E/G
-
Aquifex aeolicus
Rng
-
Aquifex aeolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 60 recombinant enzyme Aquifex aeolicus
45 75 assay at, the cleavage site selection of RNase E/G is temperature-dependent, overview Aquifex aeolicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 85 recombinant enzyme Aquifex aeolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Aquifex aeolicus