Cloned (Comment) | Organism |
---|---|
TrZL1 cloned into pBluescript KS II, subcloned into the NotI/XhoI sites of pET29 and expressed in Escherichia coli BL21-AI cells | Arabidopsis thaliana |
TrZS1 expressed as described previously. TrZL2 cloned into pBluescript KS II, subcloned into the pET29 expression vector previously digested with BamHI/XhoI and expressed in Escherichia coli Rosetta cells | Arabidopsis thaliana |
TrZS2 cloned into the pUC18 vector, subcloned into the NcoI/XhoI sites of pET32 expression vector and expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells as a fusion protein with both His and S tags | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
cytoplasm | TrZS1 | Arabidopsis thaliana | 5737 | - |
mitochondrion | TrZL2 | Arabidopsis thaliana | 5739 | - |
nucleus | - |
Arabidopsis thaliana | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana | Q8L633 | - |
- |
Arabidopsis thaliana | Q8LGU7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant TrZS1 purified. TrZL2 protein purified using the S tag | Arabidopsis thaliana |
TrZL1 protein purified using the S tag | Arabidopsis thaliana |
TrZS2 protein purified using the S tag | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | mRNAs corresponding to the tRNase Z proteins are found in all plant tissues. No significant variation between mRNA levels for TrZS1 and TrZL2 | Arabidopsis thaliana | - |
additional information | mRNAs corresponding to the tRNase Z proteins are found in all plant tissues. No significant variation between mRNA levels for TrZS1, TrZS2, TrZL1 and TrZL2 | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
precursor tRNATyr + H2O | all four recombinant tRNase Z proteins have tRNA 3'-processing activity | Arabidopsis thaliana | tRNA + 3' trailer | - |
? | |
precursor tRNATyr + H2O | all four recombinant tRNase Z proteins have tRNA 3'-processing activity. TrZL2 processes the precursor less efficiently, which may be due to the fact that this enzyme is difficult to express and only low amounts of recombinant protein can be obtained | Arabidopsis thaliana | tRNA + 3' trailer | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tRNase Z | - |
Arabidopsis thaliana |
TrZL1 | - |
Arabidopsis thaliana |
TrZL2 | - |
Arabidopsis thaliana |
TrZS1 | - |
Arabidopsis thaliana |
TrZS2 | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | all tRNase Z genes are expressed during all growth stages of Arabidopsis, in particular during the first stage of development (seedling) and at the start of flowering | up |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of the chloroplastic TrZS2 gene results in an embryo-lethal phenotype | Arabidopsis thaliana |
malfunction | The knockout TrZL1 mutant shows no visible phenotype | Arabidopsis thaliana |
malfunction | The knockout TrZL2 and TrZS1 mutants show no visible phenotypes | Arabidopsis thaliana |
physiological function | Arabidopsis encodes four tRNase Z, two short forms (TrZS1 and TrZS2) and two long forms (TrZL1 and TrZL2) | Arabidopsis thaliana |
physiological function | Arabidopsis encodes four tRNase Z, two short forms (TrZS1 and TrZS2) and two long forms (TrZL1 and TrZL2). The mitochondrial tRNase Z protein TrZL2 cleaves tRNA-like elements that serve as processing signals in mitochondrial mRNA maturation | Arabidopsis thaliana |
physiological function | Arabidopsis encodes four tRNase Z, two short forms (TrZS1 and TrZS2) and two long forms (TrZL1 and TrZL2). TrZL1 is the only tRNase Z responsible for the maturation of nuclear tRNAs. The mitochondrial tRNase Z proteins TrZL1 cleave tRNA-like elements that serve as processing signals in mitochondrial mRNA maturation | Arabidopsis thaliana |