Cloned (Comment) | Organism |
---|---|
expressed in Sf-9 cells | Homo sapiens |
expression in Escherichia coli BL21(DE3) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000004 | - |
pre-tRNASer(UCN)7512G | 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens | |
0.0000048 | - |
pre-tRNASer(UCN) | 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens | |
0.0000069 | - |
pre-tRNASer(UCN)7444U | 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens | |
0.0000076 | - |
pre-tRNASer(UCN)7511G | 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens | |
0.0000108 | - |
pre-tRNASer(UCN)7510G | 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens | |
0.0000197 | - |
pre-tRNASer(UCN)7445C | 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens | |
0.000022 | - |
pre-tRNAArg | 25 mM K-MOPS, pH 6.75, 2 mM MgCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens | |
0.0000633 | - |
pre-tRNASer(UCN)7443C | 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens | |
0.0000855 | - |
pre-tRNASer(UCN)7445G | 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens | |
0.000132 | - |
pre-tRNAArg | 25 mM K-MOPS, pH 6.75, 1 mM MnCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
SDS-PAGE | Homo sapiens |
86000 | - |
SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-tag, Ni-affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNAarg + H2O | - |
Homo sapiens | ? | - |
? | |
pre-tRNASer(UCN) + H2O | wild type | Homo sapiens | ? | - |
? | |
pre-tRNASer(UCN)7443C + H2O | natural tRNA mutant | Homo sapiens | ? | - |
? | |
pre-tRNASer(UCN)7444U + H2O | natural tRNA mutant | Homo sapiens | ? | - |
? | |
pre-tRNASer(UCN)7445C + H2O | natural tRNA mutant | Homo sapiens | ? | - |
? | |
pre-tRNASer(UCN)7445G + H2O | natural tRNA mutant | Homo sapiens | ? | - |
? | |
pre-tRNASer(UCN)7510G + H2O | natural tRNA mutant | Homo sapiens | ? | - |
? | |
pre-tRNASer(UCN)7511G + H2O | natural tRNA mutant | Homo sapiens | ? | - |
? | |
pre-tRNASer(UCN)7512G + H2O | natural tRNA mutant | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(tRNase) Z | - |
Homo sapiens |
RNase Z | - |
Homo sapiens |
tRNase ZL | - |
Homo sapiens |
tRNase ZS | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
pre-tRNASer(UCN)7445C | 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens | |
0.021 | - |
pre-tRNAArg | 25 mM K-MOPS, pH 6.75, 1 mM MnCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens | |
0.05 | - |
pre-tRNASer(UCN)7512G | 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens | |
0.05 | - |
pre-tRNASer(UCN)7511G | 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens | |
0.05 | - |
pre-tRNASer(UCN)7510G | 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens | |
0.07 | - |
pre-tRNASer(UCN) | 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens | |
0.153 | - |
pre-tRNASer(UCN)7444U | 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens | |
0.23 | - |
pre-tRNASer(UCN)7443C | 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens | |
0.345 | - |
pre-tRNASer(UCN)7445G | 25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens | |
6.18 | - |
pre-tRNAArg | 25 mM K-MOPS, pH 6.75, 2 mM MgCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin | Homo sapiens |