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Literature summary for 3.1.26.10 extracted from

  • Sideris, D.C.; Fragoulis, E.G.
    Purification and characterization of a ribonuclease specific for poly(U) and poly(C) from the larvae of Ceratitis capitata (1987), Eur. J. Biochem., 164, 309-315.
    View publication on PubMed
Search for more literature for 3.1.26.10

General Stability

General Stability Organism
very unstable, DTT, GSH or 2-mercaptoethanol stabilizes Ceratitis capitata

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ 1 mM reduces activity by 15% Ceratitis capitata
additional information not inhibited by the placental RNase inhibitor Ceratitis capitata

Metals/Ions

Metals/Ions Comment Organism Structure
K+ dependent on the concentration, optimum activity at 30-50 mM Ceratitis capitata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 gel filtration Ceratitis capitata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
mRNA + H2O Ceratitis capitata control of protein synthesis in eucaryotes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Ceratitis capitata
-
 homometabolous insect
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ceratitis capitata

Source Tissue

Source Tissue Comment Organism Textmining
larva
-
 Ceratitis capitata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activity 1000000 U/mg, 1 unit is the amount of enzyme producing 0.1 A260 acid-soluble material in 30 min at 30°C under standard assay conditions Ceratitis capitata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information specifically degrades poly(U) and poly(C), fails to degrade other single-stranded homopolyribonucleotides Ceratitis capitata ?
-
 ?
mRNA + H2O control of protein synthesis in eucaryotes Ceratitis capitata ?
-
 ?
RNA + H2O RNA isolated from six-day-old larvae or rat liver Ceratitis capitata ?
-
 ?
synthetic homopolynucleotide + H2O acts on poly(U) and poly(C) with a higher affinity for the latter Ceratitis capitata ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 25000, SDS-PAGE Ceratitis capitata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 65 thermolabile, practically loses most of its activity after 10 min at 60°C, at 65°C enzyme is almost inactivated within the first min, at 99°C it is completely inactive right from the beginning Ceratitis capitata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9 Tris-HCl buffer Ceratitis capitata