Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion, both enzyme forms | Physarum polycephalum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24500 | - |
RNase Phy II, gel filtration | Physarum polycephalum |
25000 | - |
RNase Phy I, gel filtration | Physarum polycephalum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Physarum polycephalum | - |
2 enzyme forms: Rnase Phy I and RNase Phy II | - |
Purification (Comment) | Organism |
---|---|
RNase Phy I and II | Physarum polycephalum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | submersed culture | Physarum polycephalum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ApA + H2O | - |
Physarum polycephalum | 5'-AMP + adenosine | - |
? | |
ApC + H2O | - |
Physarum polycephalum | 5'-CMP + adenosine | - |
? | |
ApG + H2O | - |
Physarum polycephalum | 5'-GMP + adenosine | - |
? | |
ApU + H2O | - |
Physarum polycephalum | 5'-UMP + adenosine | - |
? | |
CpA + H2O | - |
Physarum polycephalum | 5'-AMP + cytidine | - |
? | |
CpC + H2O | - |
Physarum polycephalum | 5'-CMP + cytidine | - |
? | |
CpG + H2O | - |
Physarum polycephalum | 5'-GMP + cytidine | - |
? | |
CpU + H2O | - |
Physarum polycephalum | 5'-UMP + cytidine | - |
? | |
GpA + H2O | - |
Physarum polycephalum | 5'-AMP + guanosine | - |
? | |
GpC + H2O | - |
Physarum polycephalum | 5'-CMP + guanosine | - |
? | |
GpG + H2O | - |
Physarum polycephalum | 5'-GMP + guanosine | - |
? | |
GpU + H2O | - |
Physarum polycephalum | 5'-UMP + guanosine | - |
? | |
additional information | substrate specificity of RNase Phy I and II, the latter shows a preference for GpN substrates, while rejecting ApN dinucleotide substrates, RNase I shows a preference for ApN before GpN substrate, while CpN dinucleotides are rather resistant against hydrolysis, overview | Physarum polycephalum | ? | - |
? | |
RNA + H2O | low molecular weigt yeast RNA | Physarum polycephalum | 5'-phosphonucleotide | product identification | ? | |
UpA + H2O | - |
Physarum polycephalum | 5'-AMP + uridine | - |
? | |
UpC + H2O | - |
Physarum polycephalum | 5'-CMP + uridine | - |
? | |
UpG + H2O | - |
Physarum polycephalum | 5'-GMP + uridine | - |
? | |
UpU + H2O | - |
Physarum polycephalum | 5'-UMP + uridine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
RNase Phy I and II, assay at | Physarum polycephalum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.1 | - |
RNase Phy II, assay at | Physarum polycephalum |
4.5 | - |
RNase Phy II, assay at | Physarum polycephalum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
nactivation, RNase Phy I and II | Physarum polycephalum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Physarum polycephalum | RNase Phy I, isoelectric focusing | - |
4.2 |
Physarum polycephalum | RNase Phy II, isoelectric focusing | - |
4.2 |