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Literature summary for 3.1.25.1 extracted from
- Recognition and incision of gamma-radiation-induced cross-linked guanine-thymine tandem lesion G[8,5-Me]T by UvrABC nuclease (2005), Chem. Res. Toxicol., 18, 1339-1346.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of UvrA, UvrB, and UvrC | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | DNA-enzyme binding kinetics | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | - |
Escherichia coli |  |
| Mg2+ | - |
Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | the enzyme is involved in nucleotide excision repair of damaged DNA removing various types of bulky and distorting DNA adducts induced by UV radiation, process initiation is performed by UvrA, nicking by UvrB, which also recruits UvrC that catalyzes both the 3' and 5' incisions | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant UvrA, UvrB, and UvrC | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate | reaction mechanisms of UvrA, UvrB, and UvrC | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA with a C8 guanine adduct of N-acetyl-2-aminofluorene + H2O | - |
Escherichia coli | ? | - |
? | |
| DNA with gamma-radiation-induced cross-linked guanine-thymine tandem lesion G[8,5-Me]T + H2O | the DNA substrate contains a covalent bond between C8 of the guanine and the 5-methyl carbon of the 3'-thymine, formed by X-radiation | Escherichia coli | ? | - |
? | |
| DNA with gamma-radiation-induced cross-linked thymine-thymine lesion T[6,4]T + H2O | 6-4 pyrimidine-pyrimidone photoproduct | Escherichia coli | ? | - |
? | |
| additional information | the enzyme is involved in nucleotide excision repair of damaged DNA removing various types of bulky and distorting DNA adducts induced by UV radiation, process initiation is performed by UvrA, nicking by UvrB, which also recruits UvrC that catalyzes both the 3' and 5' incisions | Escherichia coli | ? | - |
? | |
| additional information | the enzyme is involved in nucleotide excision repair of damaged DNA removing various types of bulky and distorting DNA adducts induced by UV radiation, process initiation is performed by UvrA, nicking by UvrB, which also recruits UvrC that catalyzes both the 3' and 5' incisions, substrate recognition and reaction mechanism, overview | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UvrA | - |
Escherichia coli |
| UvrABC nuclease | - |
Escherichia coli |
| UvrB | - |
Escherichia coli |
| UvrC | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Escherichia coli |
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