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Literature summary for 3.1.25.1 extracted from

  • Latham, K.A.; Lloyd, R.S.
    T4 endonuclease V, perspectives on catalysis (1994), Ann. N. Y. Acad. Sci., 726, 181-197.
    View publication on PubMed
Search for more literature for 3.1.25.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Tequatrovirus T4

Protein Variants

Protein Variants Comment Organism
A26Q not processive Tequatrovirus T4
A3K substantial level of AP- and dimer specific nicking, not processive Tequatrovirus T4
A3L substantial level of AP- and dimer specific nicking, not processive Tequatrovirus T4
A3Q substantial level of AP- and dimer specific nicking, not processive Tequatrovirus T4
E23D no detectable N-glycosylase activity on pyrimidine dimers, partial retention of AP-lyase activity Tequatrovirus T4
E23Q no detectable N-glycosylase activity on pyrimidine dimers, loss of AP-lyase activity Tequatrovirus T4
G133D impaired ability to bind dimer-containing DNA Tequatrovirus T4
K130G low dimer-specific binding activity, AP-endonuclease function several times as efficient as that of the wild-type Tequatrovirus T4
K130H low dimer-specific binding activity, wild-type lyase activity, reduced glycosylase activity Tequatrovirus T4
K33Q in combination with A26Q, not processive Tequatrovirus T4
additional information insertion of Gly between the first 2 amino acids, lack of dimer-specific glycosylase and nicking activity Tequatrovirus T4
T2P lack of dimer-specific glycosylase and nicking activity Tequatrovirus T4
T2S wild-type level of dimer-specific glycosylase and nicking activity Tequatrovirus T4
T2V intermediate activity level of dimer-specific glycosylase and nicking activity Tequatrovirus T4
W128S wild-type glycosylase but virtually no AP-lyase activity Tequatrovirus T4
Y129N
-
 Tequatrovirus T4
Y131N in combination with Y129N, wild-type lyase activity retained, diminished dimer-specific binding and N-glycosylase activity Tequatrovirus T4

Inhibitors

Inhibitors Comment Organism Structure
CH2O total inhibition if 4.4 methylations per molecule are present Tequatrovirus T4
NaBH4 leads to a covalently linked enzyme-substrate product Tequatrovirus T4
NaCN reacts with imino intermediate Tequatrovirus T4
NaCNBH3 total inhibition if 4.4 methylations per molecule are present Tequatrovirus T4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
-
 Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Tequatrovirus T4
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification methylation, after treatment with CH2O and NaCNBH3, N-terminal alpha-amino group is preferentially methylated, glycosylase and endonuclease activity are reduced to 20% if 0.8 methylations are present per enzyme molecule, with 4.4 methyl groups per enzyme both nicking and DNA-binding are abolished Tequatrovirus T4

Purification (Commentary)

Purification (Comment) Organism
-
 Tequatrovirus T4

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate specific for cis-syn cyclobutane pyrimidine dimers Tequatrovirus T4
a
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate cleavage on the 5'-side of dimers Tequatrovirus T4
a
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate salt concentrations of more than 40 mM cause the endonuclease V to shift into a distributive search mechanism instead of the processive mechanism Tequatrovirus T4
a
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate 2-step mechanism, pyrimidine dimer-DNA glycosylase and an apurinic/apyrimidinic endonuclease Tequatrovirus T4
a
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate processive Tequatrovirus T4
a
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate beta-elimination mechanism, imino intermediate Tequatrovirus T4
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
 Tequatrovirus T4 ?
-
 ?
additional information not: unirradiated or heat-denatured DNA Tequatrovirus T4 ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8.5 pH-optimum of glycosylase activity Tequatrovirus T4
6.5
-
 pH-optimum of endonuclease activity Tequatrovirus T4