Reference to 3.1.25.1; Id = 170712 - BRENDA Enzyme Database

p-chloromercuribenzoate

70-80% inhibition in presence of 0.0004 M

Escherichia virus T4

p-chloromercuriphenylsulfonic acid

20-25% inhibition in presence of 0.0001 M

Escherichia virus T4

Mg2+

slight stimulation

Escherichia virus T4

Mn2+

slight stimulation

Escherichia virus T4

18000

-

PAGE, gel filtration

Escherichia virus T4

DNA + H2O

Escherichia virus T4

-

?

-

?

Escherichia virus T4

-

100-300fold, extensive purification, attempts to concentrate the enzyme preparation by Aquacide II or Sephadex G50 powder results in loss of activity, UV-DNA-cellulose chromatography, hydroxyapatite chromatography, gel filtration through Sephadex or CM-Sephadex as well as sedimentation in sucrose density gradients results in loss of activity if used after phosphocellulose chromatography, enzyme is more stable in fractions containing large amounts of protein like bovine serum albumin

Escherichia virus T4

infected cell

T4 infected

Escherichia virus T4

-

-12Â°C, in 12% glycerol, inactivates the enzyme

Escherichia virus T4

4Â°C, in 50 mM Tris-HCl, pH 7.5, containing 10% ethylene glycol or 3% polyethylene glycol, storage for several months

Escherichia virus T4

freezing, leads to inactivation

Escherichia virus T4

ColE1 DNA + H2O

UV-irradiated, superhelical

170712

Escherichia virus T4

oligonucleotides

-

170712

Escherichia virus T4

?

DNA + H2O

-

170712

Escherichia virus T4

?

-

?

E. coli DNA + H2O

single-stranded DNA

170712

Escherichia virus T4

oligonucleotides

-

170712

Escherichia virus T4

?

E. coli DNA + H2O

UV-irradiated dsDNA

170712

Escherichia virus T4

oligonucleotides

-

170712

Escherichia virus T4

?

glycosylated DNA + H2O

UV-irradiated, cleaves also nonglycosylated DNA

170712

Escherichia virus T4

oligonucleotides

-

170712

Escherichia virus T4

?

additional information

not: 5-thyminyl-5,6-dihydrothymine-containing DNA

170712

Escherichia virus T4

?

-

?

additional information

not: DNA exposed to ionizing radiation, methyl methane sulfonate, nitrogen mustard, mitomycin C, 4-nitroquinoline-1-oxide

170712

Escherichia virus T4

?

-

?

PBS1 DNA

UV irradiated, contains uracil instead of thymine

170712

Escherichia virus T4

oligonucleotides

-

170712

Escherichia virus T4

?

SPO1 DNA

UV irradiated, contains 5-hydroxymethyluracil instead of thymine

170712

Escherichia virus T4

oligonucleotides

-

170712

Escherichia virus T4

?

superhelical DNA + H2O

UV-irradiated, cleaves also linear DNA

170712

Escherichia virus T4

oligonucleotides

-

170712

Escherichia virus T4

?

p-chloromercuribenzoate

70-80% inhibition in presence of 0.0004 M

Escherichia virus T4

p-chloromercuriphenylsulfonic acid

20-25% inhibition in presence of 0.0001 M

Escherichia virus T4

Mg2+

slight stimulation

Escherichia virus T4

Mn2+

slight stimulation

Escherichia virus T4

18000

-

PAGE, gel filtration

Escherichia virus T4

DNA + H2O

Escherichia virus T4

-

?

-

?

100-300fold, extensive purification, attempts to concentrate the enzyme preparation by Aquacide II or Sephadex G50 powder results in loss of activity, UV-DNA-cellulose chromatography, hydroxyapatite chromatography, gel filtration through Sephadex or CM-Sephadex as well as sedimentation in sucrose density gradients results in loss of activity if used after phosphocellulose chromatography, enzyme is more stable in fractions containing large amounts of protein like bovine serum albumin

Escherichia virus T4

infected cell

T4 infected

Escherichia virus T4

-

-12Â°C, in 12% glycerol, inactivates the enzyme

Escherichia virus T4

4Â°C, in 50 mM Tris-HCl, pH 7.5, containing 10% ethylene glycol or 3% polyethylene glycol, storage for several months

Escherichia virus T4

freezing, leads to inactivation

Escherichia virus T4

ColE1 DNA + H2O

UV-irradiated, superhelical

170712

Escherichia virus T4

oligonucleotides

-

170712

Escherichia virus T4

?

DNA + H2O

-

170712

Escherichia virus T4

?

-

?

E. coli DNA + H2O

single-stranded DNA

170712

Escherichia virus T4

oligonucleotides

-

170712

Escherichia virus T4

?

E. coli DNA + H2O

UV-irradiated dsDNA

170712

Escherichia virus T4

oligonucleotides

-

170712

Escherichia virus T4

?

glycosylated DNA + H2O

UV-irradiated, cleaves also nonglycosylated DNA

170712

Escherichia virus T4

oligonucleotides

-

170712

Escherichia virus T4

?

additional information

not: 5-thyminyl-5,6-dihydrothymine-containing DNA

170712

Escherichia virus T4

?

-

?

additional information

not: DNA exposed to ionizing radiation, methyl methane sulfonate, nitrogen mustard, mitomycin C, 4-nitroquinoline-1-oxide

170712

Escherichia virus T4

?

-

?

PBS1 DNA

UV irradiated, contains uracil instead of thymine

170712

Escherichia virus T4

oligonucleotides

-

170712

Escherichia virus T4

?

SPO1 DNA

UV irradiated, contains 5-hydroxymethyluracil instead of thymine

170712

Escherichia virus T4

oligonucleotides

-

170712

Escherichia virus T4

?

superhelical DNA + H2O

UV-irradiated, cleaves also linear DNA

170712

Escherichia virus T4

oligonucleotides

-

170712

Escherichia virus T4

?

710256

Hacker

Enhancement of DNA repair usin ...

Mus musculus

Pigment Cell Melanoma Res.

23

121-128

2010

-

10

-

2

-

2

-

2

-

2

-

691338

Jiang

UVA generates pyrimidine dimer ...

Escherichia coli

Biophys. J.

96

1151-1158

2009

-

1

-

4

-

4

-

1

-

4

-

1

-

1

-

4

-

1

-

4

-

1

-

663864

Kim Sang

Characterization of a wide ran ...

Homo sapiens, Mus musculus

Biochem. Biophys. Res. Commun.

328

962-967

2005

-

1

-

3

-

2

-

2

-

2

-

2

6

2

9

2

-

2

-

2

-

1

-

3

-

2

-

2

-

2

6

2

-

2

-

2

-

664093

Minko

Initiation of repair of DNA-po ...

[Bacillus] caldotenax, Thermotoga maritima

Biochemistry

44

3000-3009

2005

-

2

-

2

-

2

-

4

-

1

-

4

-

5

2

-

2

-

2

-

2

-

2

-

1

-

4

-

2

-

2

-

664112

Feng

Active site plasticity of endo ...

Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium 14028

Biochemistry

44

675-683

2005

-

1

-

1

-

4

-

2

-

2

-

1

-

6

-

2

1

-

1

-

1

-

1

-

4

-

2

-

1

-

6

-

1

-

1

-

664594

Yang

Recognition and incision of ga ...

Escherichia coli

Chem. Res. Toxicol.

18

1339-1346

2005

-

1

-

1

-

2

-

1

-

2

-

1

-

5

-

4

1

-

1

-

1

-

1

-

2

-

1

-

1

-

5

-

1

-

1

-

666546

Kuchel

Cyclobutane pyrimidine dimer f ...

Escherichia virus T4

Photochem. Photobiol. Sci.

4

577-582

2005

-

1

-

1

-

5

-

2

-

3

-

1

-

1

-

2

-

656881

Takasawa

Chemical synthesis and transle ...

Escherichia virus T4

Nucleic Acids Res.

32

1738-1745

2004

-

1

-

2

-

2

-

2

1

-

1

-

1

-

2

-

1

-

1

-

656151

Ahn

Interaction of T4 endonuclease ...

Escherichia virus T4

J. Biol. Chem.

278

30985-30992

2003

-

1

-

1

-

1

-

2

1

2

-

1

-

2

1

-

656845

Yarosh

Enhanced DNA repair of cyclobu ...

Escherichia virus T4

Mutat. Res.

509

221-226

2002

-

2

-

1

-

3

-

2

-

1

-

2

-

1

-

2

-

656844

Morikawa

Three-dimensional structural v ...

Escherichia virus T4

Mutat. Res.

460

257-275

2000

-

1

3

-

1

-

7

-

1

-

2

1

4

-

1

-

1

3

-

1

-

1

-

2

1

-

170716

Shiota

UV endonuclease of Micrococcus ...

Micrococcus luteus

Proc. Natl. Acad. Sci. USA

94

593-598

1997

-

1

-

2

1

-

1

-

1

-

2

-

1

-

2

1

-

1

-

2

-

170713

Valerie

Replacing tryptophan-128 of T4 ...

Escherichia virus T4

Nucleic Acids Res.

23

3764-3770

1995

-

1

-

9

-

1

2

-

1

-

2

-

1

-

1

-

1

-

2

-

170717

Piersen

Purification and cloning of Mi ...

Micrococcus luteus

J. Biol. Chem.

270

23475-23484

1995

-

1

-

2

-

1

-

11

-

1

3

-

3

-

1

-

4

-

1

-

1

-

3

-

170714

Latham

T4 endonuclease V, perspective ...

Escherichia virus T4

Ann. N. Y. Acad. Sci.

726

181-197

1994

-

1

-

17

-

4

-

1

-

1

-

1

6

-

2

-

2

-

1

-

17

-

4

-

1

-

1

-

2

-

2

-

170715

Kibitel

Enhancment of ultraviolet-DNA ...

Escherichia virus T4

Photochem. Photobiol.

54

753-760

1991

-

1

-

1

-

7

-

2

-

1

-

1

-

1

-

2

-

1

-

170703

Ishida

In vitro and in vivo activitie ...

Escherichia virus T4

Biochemistry

29

3817-3821

1990

-

8

1

-

1

-

1

-

9

-

1

2

1

-

3

-

2

-

8

1

-

1

-

1

-

1

-

3

-

2

-

170700

Dowd

Site-directed mutagenesis of t ...

Escherichia virus T4

Biochemistry

28

8699-8705

1989

-

1

-

6

-

1

-

7

-

3

-

1

-

1

-

6

-

1

-

1

-

170702

Inaoka

Affinity of single- or double- ...

Escherichia virus T4

J. Biol. Chem.

264

2609-2614

1989

-

1

-

2

-

1

-

3

-

1

-

4

-

2

-

1

-

2

-

1

-

1

-

4

-

2

-

170697

Recinos

Site-directed mutagenesis of t ...

Escherichia virus T4

Biochemistry

27

1832-1838

1988

-

1

-

3

-

1

-

7

-

2

-

1

4

-

1

-

3

-

1

-

1

4

-

170698

Stump

Site-directed mutagenesis of t ...

Escherichia virus T4

Biochemistry

27

1839-1843

1988

-

1

-

2

-

1

-

3

-

1

-

3

-

1

-

2

-

1

-

3

-

170699

Morikawa

Preliminary crystallographic s ...

Escherichia virus T4

J. Mol. Biol.

202

683-684

1988

-

1

-

1

4

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

170701

Gruskin

Molecular analysis of plasmid ...

Escherichia virus T4

J. Biol. Chem.

263

12728-12737

1988

-

1

-

3

-

3

-

1

-

2

-

1

-

1

-

2

-

170694

Nakabeppu

Purification and characterizat ...

Escherichia virus T4

J. Biol. Chem.

257

2556-2562

1982

-

1

-

2

1

-

5

-

1

-

1

2

-

3

-

1

-

2

1

-

1

-

1

2

-

3

-

170695

Nakatsu

Action of T4 endonuclease V on ...

Escherichia virus T4

J. Biochem.

91

2057-2065

1982

-

2

-

1

-

1

-

1

2

-

1

-

3

-

2

-

1

-

1

-

1

-

3

-

170706

Nakabeppu

Physical association of pyrimi ...

Escherichia virus T4

Proc. Natl. Acad. Sci. USA

78

2742-2746

1981

-

1

-

1

-

5

-

1

2

-

4

-

2

-

2

-

1

-

1

-

1

-

4

-

2

-

2

-

170711

Riazuddin

Purification and properties of ...

Micrococcus luteus

Methods Enzymol.

65

185-191

1980

2

-

3

-

1

-

1

-

1

-

4

-

1

4

-

1

-

1

-

2

-

3

-

1

-

1

-

1

4

-

1

-

1

-

170712

Friedberg

Purification and properties of ...

Escherichia virus T4

Methods Enzymol.

65

191-201

1980

-

2

-

2

1

-

1

-

1

-

1

-

3

10

-

2

-

2

1

-

1

-

1

-

3

10

-

170704

Makino

Action of T4 endonuclease V on ...

Escherichia virus T4

J. Biochem.

82

1567-1573

1977

-

1

-

5

-

1

-

2

-

5

-

1

-

1

-

2

-

5

-

170707

Riazuddin

Micrococcus luteus correndonuc ...

Micrococcus luteus

J. Biol. Chem.

252

6280-6286

1977

3

-

6

-

3

1

-

2

-

1

-

1

3

4

-

1

-

1

-

3

-

6

-

3

1

-

1

-

1

3

4

-

1

-

1

-

170708

Riazuddin

Micrococcus luteus correndonuc ...

Micrococcus luteus

J. Biol. Chem.

252

6287-6293

1977

-

1

-

1

-

3

-

7

-

1

-

7

-

170709

Riazuddin

Micrococcus luteus correndonuc ...

Micrococcus luteus

J. Biol. Chem.

252

6294-6298

1977

-

1

-

1

-

1

-

2

-

1

-

2

-

170705

Sato

Studies on temperature-depende ...

Escherichia virus T4

J. Mol. Biol.

102

15-26

1976

-

1

-

3

-

1

-

2

-

1

-

2

-

1

-

1

-

2

-

1

-

2

-

170710

Braun

Enzymatic repair of DNA: Sites ...

Escherichia coli

Biochemistry

15

4116-4120

1976

-

1

-

1

-

1

-

1

3

-

1

-

1

-

1

3

-

170696

Minton

The ultraviolet endonuclease o ...

Escherichia virus T4

J. Biol. Chem.

250

2823-2829

1975

-

1

-

2

1

-

1

-

1

-

1

5

-

1

-

1

-

2

1

-

1

-

1

5

-

1

-

Purification and properties of a pyrimidine dimer-specific endonuclease from E. coli infected with bacteriophage T4

Data extracted from this reference: