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Literature summary for 3.1.25.1 extracted from

  • Riazuddin, S.; Grossman, L.
    Micrococcus luteus correndonucleases. I. resolution and purification of two endonucleases specific for DNA containing pyrimidine dimers (1977), J. Biol. Chem., 252, 6280-6286.
    View publication on PubMed
Search for more literature for 3.1.25.1

Activating Compound

Activating Compound Comment Organism Structure
rATP stimulates Py-Py correndonuclease I activity Micrococcus luteus
Tris-HCl optimum for Py-Py correndonuclease I: 10 mM supplemented with 50 mM NaCl or potassium phosphate Micrococcus luteus
Tris-HCl optimum for Py-Py correndonuclease II: 10 mM Micrococcus luteus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ inhibits both Py-Py correndonucleases Micrococcus luteus
EDTA inhibits Py-Py correndonuclease II at concentrations above 20 mM Micrococcus luteus
KCl Py-Py correndonuclease II loses 75% of activity in presence of 100 mM Micrococcus luteus
Mg2+ Py-Py correndonuclease II loses 75% of activity in presence of 15 mM Micrococcus luteus
NaCl Py-Py correndonuclease II loses 75% of activity in presence of 100 mM Micrococcus luteus
Zn2+ inhibits both Py-Py correndonucleases Micrococcus luteus

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl activating Micrococcus luteus
NaCl optimum for Py-Py correndonuclease I: 50 mM supplemented with 10 mM Tris-HCl Micrococcus luteus
potassium phosphate activating, optimum for Py-Py correndonuclease I: 50 mM supplemented with 10 mM Tris-HCl Micrococcus luteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
11000 15000 Py-Py correndonucleases I and II Micrococcus luteus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Micrococcus luteus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Micrococcus luteus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Micrococcus luteus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Micrococcus luteus

Storage Stability

Storage Stability Organism
-20°C, in 50% glycerol, complete inactivation in 3-4 weeks Micrococcus luteus
in liquid nitrogen, for minimal inactivation Micrococcus luteus
in liquid nitrogen, for minimal inactivation, freeze-thawing does not inactivate either Py-Py correndonuclease Micrococcus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
 Micrococcus luteus ?
-
 ?
additional information not: x-irradiated, psoralen-crosslinked or alkylated DNA Micrococcus luteus ?
-
 ?
X174 DNA + H2O UV-irradiated, denatured DNA: substrate for Py-Py correndonuclease I only Micrococcus luteus RFII
-
 ?
X174 DNA + H2O UV irradiated RFI of phage X174 containing apurinic sites Micrococcus luteus RFII
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 Py-Py correndonuclease I loses 20% of activity after 5 min, Py-Py correndonuclease II loses more than 75% of activity after 5 min Micrococcus luteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.4 Py-Py correndonucleases I and II Micrococcus luteus

pH Range

pH Minimum pH Maximum Comment Organism
4 10 Py-Py correndonucleases I and II, no activity below or above these values Micrococcus luteus