Application | Comment | Organism |
---|---|---|
analysis | - |
Tequatrovirus T4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
SDS-PAGE, gel filtration | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Tequatrovirus T4 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity | Tequatrovirus T4 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate | cleavage on the 5'-side of dimers | Tequatrovirus T4 | |
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate | 2-step mechanism, pyrimidine dimer-DNA glycosylase and an apurinic/apyrimidinic endonuclease | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Tequatrovirus T4 | ? | - |
? | |
additional information | not: DNA damaged by 4-nitroquinoline-N-oxide or methyl methanesulfonate | Tequatrovirus T4 | ? | - |
? | |
poly(dA)-poly(dT) + H2O | UV-irradiated | Tequatrovirus T4 | oligonucleotides | - |
? | |
T4 DNA | UV-irradiated | Tequatrovirus T4 | oligonucleotides | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
glycosylase activity decreases linearly: loss of 4% of activity per min | Tequatrovirus T4 |
42 | - |
endonuclease activity decreases by 50% after 3 min, after 20 min ca. 25% of activity remains | Tequatrovirus T4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | pH-optimum of glycosylase activity | Tequatrovirus T4 |
6.5 | - |
pH-optimum of endonuclease activity | Tequatrovirus T4 |