Protein Variants | Comment | Organism |
---|---|---|
DELTAC127 | C127-truncated, total loss of activity | Tequatrovirus T4 |
K130L | almost same activity as wild-type | Tequatrovirus T4 |
T127M | almost same activity as wild-type, Km value in the same order as that of wild-type | Tequatrovirus T4 |
W128A | total loss of activity | Tequatrovirus T4 |
W128S | only slight activity remains | Tequatrovirus T4 |
Y129A | total loss of activity | Tequatrovirus T4 |
Y131A | only slight activity remains | Tequatrovirus T4 |
Y132A | only slight activity remains | Tequatrovirus T4 |
General Stability | Organism |
---|---|
addition of 8 M urea leads to denaturation | Tequatrovirus T4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000015 | - |
DNA 14mer containing a TT-dimer | WT-enzyme and T127M | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Tequatrovirus T4 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
T127M | Tequatrovirus T4 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate | cleavage on the 5'-side of dimers | Tequatrovirus T4 | |
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate | 2-step mechanism, pyrimidine dimer-DNA glycosylase and an apurinic/apyrimidinic endonuclease | Tequatrovirus T4 |
Renatured (Comment) | Organism |
---|---|
- |
Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Tequatrovirus T4 | ? | - |
? | |
DNA 14mer containing a TT-dimer + H2O | - |
Tequatrovirus T4 | ? | - |
? | |
oligodeoxyribonucleotides | 14mer containing a TT-dimer, duplex | Tequatrovirus T4 | oligonucleotides | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00833 | - |
DNA 14mer containing a TT-dimer | T127M | Tequatrovirus T4 | |
0.0183 | - |
DNA 14mer containing a TT-dimer | WT-enzyme | Tequatrovirus T4 |