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Literature summary for 3.1.25.1 extracted from

  • Dowd, D.R.; Lloyd, R.S.
    Site-directed mutagenesis of the T4 endonuclease V gene: the role of arginine-3 in the target search (1989), Biochemistry, 28, 8699-8705.
    View publication on PubMed
Search for more literature for 3.1.25.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Tequatrovirus T4

Protein Variants

Protein Variants Comment Organism
A3D AP-specific nicking possible, low level of dimer specific nicking Tequatrovirus T4
A3E AP-specific nicking possible, low level of dimer specific nicking Tequatrovirus T4
A3K substantial level of AP- and dimer specific nicking, not processive Tequatrovirus T4
A3L substantial level of AP- and dimer specific nicking, not processive Tequatrovirus T4
A3Q substantial level of AP- and dimer specific nicking, not processive Tequatrovirus T4
additional information deletion of Arg 3, only marginal level of AP specificity, no other detectable activity Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Tequatrovirus T4
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate cleavage on the 5'-side of dimers Tequatrovirus T4
a
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate 2-step mechanism, pyrimidine dimer-DNA glycosylase and an apurinic/apyrimidinic endonuclease Tequatrovirus T4
a
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate processive Tequatrovirus T4
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
 Tequatrovirus T4 ?
-
 ?