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Literature summary for 3.1.25.1 extracted from
- Site-directed mutagenesis of the T4 endonuclease V gene: role of tyrosine-129 and -131 in pyrimidine dimer-specific binding (1988), Biochemistry, 27, 1839-1843.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Tequatrovirus T4 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Y129N | dimer-specific binding and nicking abolished, apurinic-specific nicking abolished | Tequatrovirus T4 |
| Y131N | in combination with Y129N, dimer-specific binding and nicking abolished, ability for apurinic-specific nicking retained | Tequatrovirus T4 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | Tequatrovirus T4 | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tequatrovirus T4 | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate | 2-step mechanism, pyrimidine dimer-DNA glycosylase and an apurinic/apyrimidinic endonuclease | Tequatrovirus T4 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | - |
Tequatrovirus T4 | ? | - |
? | |
| native DNA + H2O | E. coli DNA in vivo | Tequatrovirus T4 | oligonucleotides | - |
? | |
| pBR322 DNA + H2O | UV-irradiated | Tequatrovirus T4 | oligonucleotides | - |
? | |
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