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Literature summary for 3.1.25.1 extracted from

  • Recinos , A.; Lloyd, R.S.
    Site-directed mutagenesis of the T4 endonuclease V gene: role of lysine-130 (1988), Biochemistry, 27, 1832-1838.
    View publication on PubMed
Search for more literature for 3.1.25.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Tequatrovirus T4

Protein Variants

Protein Variants Comment Organism
K130G diminished capacity to make complete incisions, significantly decreased intracellular accumulation Tequatrovirus T4
K130G low dimer-specific binding activity, AP-endonuclease function several times as efficient as that of the wild-type Tequatrovirus T4
K130H diminished capacity to make complete incisions, slight decreased intracellular accumulation Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Tequatrovirus T4
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate cleavage on the 3'-side of the apyrimidinic site Tequatrovirus T4
a
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate 2-step mechanism, pyrimidine dimer-DNA glycosylase and an apurinic/apyrimidinic endonuclease Tequatrovirus T4
a

Storage Stability

Storage Stability Organism
4°C, stock enzyme-containing cell extracts Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
 Tequatrovirus T4 ?
-
 ?
E. coli DNA + H2O
-
 Tequatrovirus T4 oligonucleotides
-
 ?
native DNA + H2O E. coli DNA in vivo Tequatrovirus T4 oligonucleotides
-
 ?
pBR322 DNA + H2O UV-irradiated Tequatrovirus T4 oligonucleotides
-
 ?