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Literature summary for 3.1.25.1 extracted from
- Purification and characterization of normal and mutant forms of T4 endonuclease V (1982), J. Biol. Chem., 257, 2556-2562.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | inhibits endonuclease activity | Tequatrovirus T4 |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | activating | Tequatrovirus T4 | |
| NaCl | optimum for the glycosylase: 25-100 mM | Tequatrovirus T4 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16000 | - |
SDS-PAGE | Tequatrovirus T4 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | Tequatrovirus T4 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tequatrovirus T4 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 4100fold, to homogeneity | Tequatrovirus T4 |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate | 2-step mechanism, pyrimidine dimer-DNA glycosylase and an apurinic/apyrimidinic endonuclease | Tequatrovirus T4 |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| infected cell | T4 infected | Tequatrovirus T4 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Tequatrovirus T4 |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, addition of an equal value of glycerol | Tequatrovirus T4 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | - |
Tequatrovirus T4 | ? | - |
? | |
| poly(dA)-poly(dT) + H2O | UV-irradiated | Tequatrovirus T4 | oligonucleotides | length is less than 15 nucleotides | ? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
pH-optimum of endonuclease activity | Tequatrovirus T4 |
| 6 | 8.5 | pH-optimum of glycosylase activity | Tequatrovirus T4 |
| 6.5 | - |
pH-optimum of endonuclease activity | Tequatrovirus T4 |
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