Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Escherichia coli | |
ATP | - |
Escherichia coli | |
Ca2+ | at concentrations above 1.5 mM | Escherichia coli | |
Mg2+ | at concentrations above 5 mM | Escherichia coli | |
additional information | no effect: adenosine, thymidine, RNA and double-stranded DNA | Escherichia coli | |
single-stranded DNA | - |
Escherichia coli | |
Tris-HCl | optimum concentration at 10-150 mM, inhibition near 150 mM | Escherichia coli | |
TTP | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | absolute requirement for a divalent cation, optimum concentration: 1.5 mM, inhibition at higher concentrations | Escherichia coli | |
Mg2+ | absolute requirement for a divalent cation, optimum concentration: 5 mM, inhibition at higher concentrations | Escherichia coli | |
additional information | very little activity with Mn2+ or Zn2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
x * 49000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
supercoiled plasma DNA + H2O | Escherichia coli | - |
linear DNA duplexes | - |
? | |
supercoiled plasma DNA + H2O | Escherichia coli B / ATCC 11303 | - |
linear DNA duplexes | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linear double-stranded DNA + H2O | little activity | Escherichia coli | ? | - |
? | |
linear double-stranded DNA + H2O | little activity | Escherichia coli B / ATCC 11303 | ? | - |
? | |
supercoiled plasma DNA + H2O | - |
Escherichia coli | linear DNA duplexes | - |
? | |
supercoiled plasma DNA + H2O | enzyme recognizes cruziforme structure in supercoiled plasmid DNA, e.g. pBR322 form I DNA is first converted to relaxed circular form II-dimer and subsequently to linear form III-dimer of pBR322, ColE-1 DNA is converted to linear products, the enzyme appears to cleave at more than one site on superhelical pBR322 DNA, a double-strand break at one site seems to block further double-strand cleavage in the DNA molecule | Escherichia coli | linear DNA duplexes | - |
? | |
supercoiled plasma DNA + H2O | not: lambda DNA | Escherichia coli | linear DNA duplexes | - |
? | |
supercoiled plasma DNA + H2O | - |
Escherichia coli B / ATCC 11303 | linear DNA duplexes | - |
? | |
supercoiled plasma DNA + H2O | enzyme recognizes cruziforme structure in supercoiled plasmid DNA, e.g. pBR322 form I DNA is first converted to relaxed circular form II-dimer and subsequently to linear form III-dimer of pBR322, ColE-1 DNA is converted to linear products, the enzyme appears to cleave at more than one site on superhelical pBR322 DNA, a double-strand break at one site seems to block further double-strand cleavage in the DNA molecule | Escherichia coli B / ATCC 11303 | linear DNA duplexes | - |
? | |
supercoiled plasma DNA + H2O | not: lambda DNA | Escherichia coli B / ATCC 11303 | linear DNA duplexes | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, SDS-PAGE | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.8 | pBR322 | Escherichia coli |