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Literature summary for 3.1.22.1 extracted from

  • Varela-Ramirez, A.; Abendroth, J.; Mejia, A.A.; Phan, I.Q.; Lorimer, D.D.; Edwards, T.E.; Aguilera, R.J.
    Structure of acid deoxyribonuclease (2017), Nucleic Acids Res., 45, 6217-6227 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Burkholderia thailandensis

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of BthDNase II grown from a solution containing 0.0004 ml of protein at 26.5 mg/ml and 0.0004 ml of precipitant equilibrated against 0.080 ml of precipitant (100 mM BisTris pH 6.5, 28% (w/v) PEG MME 2000). A single crystal is cryo-protected with 15% (v/v) ethylene glycol and vitrified in liquid nitrogen. Crystal structure solved at 1.65 A resolution Burkholderia thailandensis

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ inhibited by copper ions and to a lesser extent manganese ions, but not inhibited by calcium, magnesium or zinc ions Burkholderia thailandensis
Mn2+ inhibited by copper ions and to a lesser extent manganese ions, but not inhibited by calcium, magnesium or zinc ions Burkholderia thailandensis

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40301
-
calculated from sequence Burkholderia thailandensis

Organism

Organism UniProt Comment Textmining
Burkholderia thailandensis Q2T8B0
-
-
Burkholderia thailandensis ATCC 700388 Q2T8B0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Burkholderia thailandensis

Subunits

Subunits Comment Organism
dimer
-
Burkholderia thailandensis

Synonyms

Synonyms Comment Organism
DNase II
-
Burkholderia thailandensis

pI Value

Organism Comment pI Value Maximum pI Value
Burkholderia thailandensis calculated from sequence
-
7