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Literature summary for 3.1.22.1 extracted from

  • Krasnorutskii, M.A.; Buneva, V.N.; Nevinsky, G.A.
    Immunization of rabbits with DNase II leads to formation of polyclonal antibodies with DNase and RNase activities (2009), Int. Immunol., 21, 349-360.
    View publication on PubMed
Search for more literature for 3.1.22.1

Application

Application Comment Organism
medicine immunization of healthy rabbits with DNase II produces IgGs with intrinsic DNase and RNase activities. 10% of the total IgG DNase and RNase activities belong to anti-idiotypic antibodies to DNase II (ca. 0.1% of total pIgGs), while 90% of the activities do not interact with Sepharose bearing antibodies against DNase II and might be antibodies to nucleic acids bound to DNase II. Fraction of abzymes hydrolyzing both DNA and RNA can contain subfraction of antibodies against DNase II Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
EDTA dialysis of IgGs from rabbits immunized with DNase II against a buffer containing EDTA or addition of EDTA to the reaction mixture leads to a complete disappearance of DNase activity Bos taurus
Mg2+ addition of Mg2+ ions to the dialyzed preparations of IgGs leads to a reduction of the IgG DNase activity Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000005
-
 supercoiled plasmid DNA pH 2.6, 3.0% IgG relative content, 18.6% relative DNase activity Bos taurus
0.0000008
-
 supercoiled plasmid DNA 3 M NaCl, 2.0% IgG relative content, 22.3% relative DNase activity Bos taurus
0.0000135
-
 supercoiled plasmid DNA 0.51 M NaCl, 28.3% IgG relative content, 27.8% relative DNase activity Bos taurus
0.0000158
-
 supercoiled plasmid DNA non-fractioned elution, 100% IgG relative content, 100% relative DNase activity Bos taurus
0.0000183
-
 supercoiled plasmid DNA 0.1 M NaCl, 11.1% IgG relative content, 25.7% relative DNase activity Bos taurus
0.0000608
-
 supercoiled plasmid DNA 0.0 M NaCl, 55.6 IgG relative content, 5.5% relative DNase activity Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ in the presence of external 5 mM Mg2+ ions, the IgGs are 17 to 25fold more active than the same IgG preparations containing intrinsic metal ions before their dialysis Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
supercoiled plasmid DNA + H2O IgGs from rabbit immunized with DNase II effectively hydrolyze DNA Bos taurus relaxed plasmid DNA + ?
-
 ?

Synonyms

Synonyms Comment Organism
DNase II
-
 Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000000183
-
 supercoiled plasmid DNA pH 2.6, 3.0% IgG relative content, 18.6% relative DNase activity Bos taurus
0.000000367
-
 supercoiled plasmid DNA 0.1 M NaCl, 11.1% IgG relative content, 25.7% relative DNase activity Bos taurus
0.00000065
-
 supercoiled plasmid DNA 3 M NaCl, 2.0% IgG relative content, 22.3% relative DNase activity Bos taurus
0.000000883
-
 supercoiled plasmid DNA 0.0 M NaCl, 55.6 IgG relative content, 5.5% relative DNase activity Bos taurus
0.00000145
-
 supercoiled plasmid DNA 0.51 M NaCl, 28.3% IgG relative content, 27.8% relative DNase activity Bos taurus
0.00000167
-
 supercoiled plasmid DNA non-fractioned elution, 100% IgG relative content, 100% relative DNase activity Bos taurus