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Literature summary for 3.1.22.1 extracted from
- Deoxyribonuclease II purified from Euglena gracilis SM-ZK, a chloroplast-lacking mutant: comparison with porcine spleen deoxyribonuclease II (2002), Comp. Biochem. Physiol. B, 131, 519-525.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not: EDTA | Euglena gracilis | |
| Na2SO4 | above 50 mM | Euglena gracilis |  |
| Na2SO4 | above 50 mM | Sus scrofa | |
| NaCl | above 250 mM | Euglena gracilis | |
| NaCl | above 250 mM | Sus scrofa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
SDS-PAGE | Euglena gracilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euglena gracilis | - |
SM-ZK | - |
| Euglena gracilis SM-ZK | - |
SM-ZK | - |
| Sus scrofa | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| the treatment with a reducing agent destroys the activity | Euglena gracilis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Euglena gracilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | double-strand and single-strand DNA, but the enzyme acts preferentially on double-strand DNA | Euglena gracilis | 3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides | this endonuclease makes double-strand breaks in the DNA substrate | ? | |
| DNA + H2O | double-strand and single-strand DNA, but the enzyme acts preferentially on double-strand DNA | Euglena gracilis SM-ZK | 3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides | this endonuclease makes double-strand breaks in the DNA substrate | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| deoxyribonuclease II | - |
Euglena gracilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Sus scrofa |
| 50 | - |
- |
Euglena gracilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable for 15 min | Euglena gracilis |
| 80 | - |
the activity completely disappears after heating for 15 min | Euglena gracilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
- |
Sus scrofa |
| 5.3 | - |
- |
Euglena gracilis |
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Release 2023.1 (January 2023)
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