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Literature summary for 3.1.22.1 extracted from

  • Harosh, I.; Binninger, D.M.; Harris, P.V.; Mezzina, M.; Boyd, J.B.
    Mechanism of action of deoxyribonuclease II from human lymphoblasts (1991), Eur. J. Biochem., 202, 479-484.
    View publication on PubMed
Search for more literature for 3.1.22.1

Inhibitors

Inhibitors Comment Organism Structure
Fe3+ complete inhibition at 0.1 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
near homogeneity by 5step chromatography techniques Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
lymphoblast
-
 Homo sapiens
-

Storage Stability

Storage Stability Organism
-20°C, 50 mM Tris-HCl, pH 7.4, 5 mM 2-mercaptoethanol, 1 mM EDTA, 1 mM PMSF, 50% glycerol v/v, several months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O preference for double-stranded DNA, terminal four 3'-nucleotides resistant to cleavage Homo sapiens 3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides prominent fragments longer than eight nucleotides ?
supercoiled plasmid DNA + H2O introduces a strong excess of single-strand nicks relative to double-strand breaks, more than 50% converted to nicked circular DNA Homo sapiens relaxed plasmid DNA + ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 irreversible loss of activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5
-
 Homo sapiens