Cloned (Comment) | Organism |
---|---|
expressed in a modified version of Escherichia coli ER 2566 (ER2566DELTAnfiDELTArnhB) where the endogenous nfi and rnhB genes have been inactivated by transformation with the kanamycin-deletion cassettes derived from the JW5547-1 (nfi::kan) and JW0178-1 (rnhB::kan) strains. For crystallisation, a site specific (D52A) truncated (residues 1-250) mutant of mEndoV is fused to His/MBP in pETM41 is expressed in BL21 CodonPlus(DE3)-RIPL Escherichia coli cells | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
- |
Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D52A | mutant enzyme totally loses the inosine-RNA incision activity | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | at pH 8.5, a Mn2+ concentration of 2.5 mM and higher inhibits activity | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | best activity at pH 8.5 and 2.5 mM Mg2+ | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q8C9A2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ACUGGACA[rI][rI]U[rI]CUCCGAGG + H2O | - |
Mus musculus | ? | - |
? | |
RNA + H2O | the enzyme is active on RNA substrates containing one or several inosines. It does not cleave at inosines in DNA | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EndoV | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
ACUGGACA[rI][rI]U[rI]CUCCGAGG | pH 7.5, 37°C | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Mus musculus |