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Literature summary for 3.1.21.7 extracted from
- EndoQ and EndoV work individually for damaged DNA base repair in Pyrococcus furiosus (2015), Biochimie, 118, 264-269.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Pyrococcus furiosus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23580 | - |
x * 23580, calculated from sequence, His-tagged enzyme | Pyrococcus furiosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxyinosine-containing deoxyoligonucleotide + H2O | Pyrococcus furiosus | the enzyme is involved in damaged DNA repair | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | Q8U263 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxyinosine-containing deoxyoligonucleotide + H2O | the enzyme is involved in damaged DNA repair | Pyrococcus furiosus | ? | - |
? | |
| deoxyinosine-containing deoxyoligonucleotide + H2O | the enzyme hydrolyzes the second phosphodiester bond 3' from deoxyinosine | Pyrococcus furiosus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23580, calculated from sequence, His-tagged enzyme | Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EndoV | - |
Pyrococcus furiosus |
| PF0987 | locus name | Pyrococcus furiosus |
| PfuEndoV | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Pyrococcus furiosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 80 | 70°C: about 50% of maximal activity, 80°C: about 35% of maximal activity | Pyrococcus furiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Pyrococcus furiosus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pyrococcus furiosus | calculated from sequence | - |
9.8 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in damaged DNA repair | Pyrococcus furiosus |
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Release 2023.1 (January 2023)
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